Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris

Sidhu VK, Vorhölter F-J, Niehaus K, Watt SA (2008)
BMC Microbiology 8(1): 87.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA
Autor*in
Sidhu, Vishaldeep K.; Vorhölter, Frank-JörgUniBi; Niehaus, KarstenUniBi; Watt, Steven A.
Abstract / Bemerkung
Background: Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e. g. the cytolysine from enterotoxic E. coli. Results: We have demonstrated that Xanthomonas campestris pv. campestris (Xcc) releases OMVs into the culture supernatant during growth. A proteome study identified 31 different proteins that associate with the OMV fraction of which half are virulence-associated. A comparison with the most abundant outer membrane ( OM) proteins revealed that some proteins are enriched in the OMV fraction. This may be connected to differences in the LPS composition between the OMVs and the OM. Furthermore, a comparison of the OMV proteomes from two different culture media indicated that the culture conditions have an impact on the protein composition. Interestingly, the proteins that are common to both culture conditions are mainly involved in virulence. Conclusion: Outer membrane vesicles released from the OM of Xcc contain membrane- and virulence-associated proteins. Future experiments will prove whether these structures can serve as "vehicles" for the transport of virulence factors into the host membrane.
Erscheinungsjahr
2008
Zeitschriftentitel
BMC Microbiology
Band
8
Ausgabe
1
Seite(n)
87
ISSN
1471-2180
Page URI
https://pub.uni-bielefeld.de/record/1587298

Zitieren

Sidhu VK, Vorhölter F-J, Niehaus K, Watt SA. Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology. 2008;8(1):87.
Sidhu, V. K., Vorhölter, F. - J., Niehaus, K., & Watt, S. A. (2008). Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology, 8(1), 87. https://doi.org/10.1186/1471-2180-8-87
Sidhu, Vishaldeep K., Vorhölter, Frank-Jörg, Niehaus, Karsten, and Watt, Steven A. 2008. “Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris”. BMC Microbiology 8 (1): 87.
Sidhu, V. K., Vorhölter, F. - J., Niehaus, K., and Watt, S. A. (2008). Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology 8, 87.
Sidhu, V.K., et al., 2008. Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology, 8(1), p 87.
V.K. Sidhu, et al., “Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris”, BMC Microbiology, vol. 8, 2008, pp. 87.
Sidhu, V.K., Vorhölter, F.-J., Niehaus, K., Watt, S.A.: Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology. 8, 87 (2008).
Sidhu, Vishaldeep K., Vorhölter, Frank-Jörg, Niehaus, Karsten, and Watt, Steven A. “Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris”. BMC Microbiology 8.1 (2008): 87.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T08:48:00Z
MD5 Prüfsumme
0bfa698c75857328ce03587420fc97ce


45 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Visualization and characterization of Pseudomonas syringae pv. tomato DC3000 pellicles.
Farias GA, Olmedilla A, Gallegos MT., Microb Biotechnol 12(4), 2019
PMID: 30838765
Highlights of the mini-symposium on extracellular vesicles in inter-organismal communication, held in Munich, Germany, August 2018.
Bielska E, Birch PRJ, Buck AH, Abreu-Goodger C, Innes RW, Jin H, Pfaffl MW, Robatzek S, Regev-Rudzki N, Tisserant C, Wang S, Weiberg A., J Extracell Vesicles 8(1), 2019
PMID: 30911363
Development of a novel S. Typhi and Paratyphi A outer membrane vesicles based bivalent vaccine against enteric fever.
Howlader DR, Koley H, Sinha R, Maiti S, Bhaumik U, Mukherjee P, Dutta S., PLoS One 13(9), 2018
PMID: 30216367
Small RNA trafficking at the forefront of plant-pathogen interactions.
Zhao Y, Liang X, Zhou JM., F1000Res 7(), 2018
PMID: 30363807
Refined annotation of the complete genome of the phytopathogenic and xanthan producing Xanthomonas campestris pv. campestris strain B100 based on RNA sequence data.
Alkhateeb RS, Rückert C, Rupp O, Pucker B, Hublik G, Wibberg D, Niehaus K, Pühler A, Vorhölter FJ., J Biotechnol 253(), 2017
PMID: 28506932
Differential accumulation of Xanthomonas campestris pv. campestris proteins during the interaction with the host plant: Contributions of an in vivo system.
Santos C, Maximiano MR, Ribeiro DG, Oliveira-Neto OB, Murad AM, Franco OL, Mehta A., Proteomics 17(12), 2017
PMID: 28471538
Bacterial outer membrane vesicles at the plant-pathogen interface.
Katsir L, Bahar O., PLoS Pathog 13(6), 2017
PMID: 28570683
Isolation of membrane vesicles from prokaryotes: a technical and biological comparison reveals heterogeneity.
Dauros Singorenko P, Chang V, Whitcombe A, Simonov D, Hong J, Phillips A, Swift S, Blenkiron C., J Extracell Vesicles 6(1), 2017
PMID: 28717421
Systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium Xanthomonas campestris.
Schatschneider S, Schneider J, Blom J, Létisse F, Niehaus K, Goesmann A, Vorhölter FJ., Microbiology 163(8), 2017
PMID: 28795660
The Type II Secreted Lipase/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines.
Nascimento R, Gouran H, Chakraborty S, Gillespie HW, Almeida-Souza HO, Tu A, Rao BJ, Feldstein PA, Bruening G, Goulart LR, Dandekar AM., Sci Rep 6(), 2016
PMID: 26753904
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis.
Ferreira RM, Moreira LM, Ferro JA, Soares MRR, Laia ML, Varani AM, de Oliveira JCF, Ferro MIT., PeerJ 4(), 2016
PMID: 26925342
LPS Remodeling Triggers Formation of Outer Membrane Vesicles in Salmonella.
Elhenawy W, Bording-Jorgensen M, Valguarnera E, Haurat MF, Wine E, Feldman MF., MBio 7(4), 2016
PMID: 27406567
In vitro Determination of Extracellular Proteins from Xylella fastidiosa.
Mendes JS, Santiago AS, Toledo MA, Horta MA, de Souza AA, Tasic L, de Souza AP., Front Microbiol 7(), 2016
PMID: 28082960
Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential roles in pathogenesis.
Fulsundar S, Kulkarni HM, Jagannadham MV, Nair R, Keerthi S, Sant P, Pardesi K, Bellare J, Chopade BA., Microb Pathog 83-84(), 2015
PMID: 25940676
Xanthomonas campestris pv. vesicatoria Secretes Proteases and Xylanases via the Xps Type II Secretion System and Outer Membrane Vesicles.
Solé M, Scheibner F, Hoffmeister AK, Hartmann N, Hause G, Rother A, Jordan M, Lautier M, Arlat M, Büttner D., J Bacteriol 197(17), 2015
PMID: 26124239
Membrane vesicle formation is associated with pyocin production under denitrifying conditions in Pseudomonas aeruginosa PAO1.
Toyofuku M, Zhou S, Sawada I, Takaya N, Uchiyama H, Nomura N., Environ Microbiol 16(9), 2014
PMID: 24112564
Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris.
Frese M, Schatschneider S, Voss J, Vorhölter FJ, Niehaus K., Arch Biochem Biophys 546(), 2014
PMID: 24508689
Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen.
Fantappiè L, de Santis M, Chiarot E, Carboni F, Bensi G, Jousson O, Margarit I, Grandi G., J Extracell Vesicles 3(), 2014
PMID: 25147647
Xylella fastidiosa outer membrane vesicles modulate plant colonization by blocking attachment to surfaces.
Ionescu M, Zaini PA, Baccari C, Tran S, da Silva AM, Lindow SE., Proc Natl Acad Sci U S A 111(37), 2014
PMID: 25197068
Interaction between mycoplasmas and plants: extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii PG8.
Chernov VM, Chernova OA, Gorshkov OV, Baranova NB, Mouzykantov AA, Nesterova TN, Ponomareva AA., Dokl Biochem Biophys 450(), 2013
PMID: 23824459
Dynamic protein phosphorylation during the growth of Xanthomonas campestris pv. campestris B100 revealed by a gel-based proteomics approach.
Musa YR, Bäsell K, Schatschneider S, Vorhölter FJ, Becher D, Niehaus K., J Biotechnol 167(2), 2013
PMID: 23792782
Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane.
Fleites LA, Mensi I, Gargani D, Zhang S, Rott P, Gabriel DW., Mol Plant Microbe Interact 26(10), 2013
PMID: 23758144
Characterization of outer membrane vesicles from Brucella melitensis and protection induced in mice.
Avila-Calderón ED, Lopez-Merino A, Jain N, Peralta H, López-Villegas EO, Sriranganathan N, Boyle SM, Witonsky S, Contreras-Rodríguez A., Clin Dev Immunol 2012(), 2012
PMID: 22242036
Selective sorting of cargo proteins into bacterial membrane vesicles.
Haurat MF, Aduse-Opoku J, Rangarajan M, Dorobantu L, Gray MR, Curtis MA, Feldman MF., J Biol Chem 286(2), 2011
PMID: 21056982
Acinetobacter baumannii secretes cytotoxic outer membrane protein A via outer membrane vesicles.
Jin JS, Kwon SO, Moon DC, Gurung M, Lee JH, Kim SI, Lee JC., PLoS One 6(2), 2011
PMID: 21386968
Extracellular Streptomyces vesicles: amphorae for survival and defence.
Schrempf H, Koebsch I, Walter S, Engelhardt H, Meschke H., Microb Biotechnol 4(2), 2011
PMID: 21342473
Pathogenomics of Xanthomonas: understanding bacterium-plant interactions.
Ryan RP, Vorhölter FJ, Potnis N, Jones JB, Van Sluys MA, Bogdanove AJ, Dow JM., Nat Rev Microbiol 9(5), 2011
PMID: 21478901
Structural analysis and involvement in plant innate immunity of Xanthomonas axonopodis pv. citri lipopolysaccharide.
Casabuono A, Petrocelli S, Ottado J, Orellano EG, Couto AS., J Biol Chem 286(29), 2011
PMID: 21596742
Regulation and secretion of Xanthomonas virulence factors.
Büttner D, Bonas U., FEMS Microbiol Rev 34(2), 2010
PMID: 19925633
Virulence and immunomodulatory roles of bacterial outer membrane vesicles.
Ellis TN, Kuehn MJ., Microbiol Mol Biol Rev 74(1), 2010
PMID: 20197500
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii.
Moreira LM, Almeida NF, Potnis N, Digiampietri LA, Adi SS, Bortolossi JC, da Silva AC, da Silva AM, de Moraes FE, de Oliveira JC, de Souza RF, Facincani AP, Ferraz AL, Ferro MI, Furlan LR, Gimenez DF, Jones JB, Kitajima EW, Laia ML, Leite RP, Nishiyama MY, Rodrigues Neto J, Nociti LA, Norman DJ, Ostroski EH, Pereira HA, Staskawicz BJ, Tezza RI, Ferro JA, Vinatzer BA, Setubal JC., BMC Genomics 11(), 2010
PMID: 20388224
Lipopolysaccharide mobility in leaf tissue of Arabidopsis thaliana.
Zeidler D, Dubery IA, Schmitt-Kopplin P, Von Rad U, Durner J., Mol Plant Pathol 11(6), 2010
PMID: 21029320
Proteome of the phytopathogen Xanthomonas citri subsp. citri: a global expression profile.
Soares MR, Facincani AP, Ferreira RM, Moreira LM, de Oliveira JC, Ferro JA, Ferro MI, Meneghini R, Gozzo FC., Proteome Sci 8(), 2010
PMID: 21062441
Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum.
Hempel J, Zehner S, Göttfert M, Patschkowski T., J Biotechnol 140(1-2), 2009
PMID: 19095018
Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots.
Rodríguez-Moreno L, Jiménez AJ, Ramos C., Microb Biotechnol 2(4), 2009
PMID: 21255279

46 References

Daten bereitgestellt von Europe PubMed Central.

International standards for naming pathovars of phytopathogenic bacteria and a list of pathovar names and pathotype strains
Dye DW, Bradbury JF, Goto M, Hayward AC, Lelliott RA, Schroth MN., 1980

Swings JG, Civerolo EL., 1993
Comparison of the genomes of two Xanthomonas pathogens with differing host specificities.
da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, do Amaral AM, Bertolini MC, Camargo LE, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Ciapina LP, Cicarelli RM, Coutinho LL, Cursino-Santos JR, El-Dorry H, Faria JB, Ferreira AJ, Ferreira RC, Ferro MI, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EG, Lemos MV, Locali EC, Machado MA, Madeira AM, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CF, Miyaki CY, Moon DH, Moreira LM, Novo MT, Okura VK, Oliveira MC, Oliveira VR, Pereira HA, Rossi A, Sena JA, Silva C, de Souza RF, Spinola LA, Takita MA, Tamura RE, Teixeira EC, Tezza RI, Trindade dos Santos M, Truffi D, Tsai SM, White FF, Setubal JC, Kitajima JP., Nature 417(6887), 2002
PMID: 12024217
The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice.
Lee BM, Park YJ, Park DS, Kang HW, Kim JG, Song ES, Park IC, Yoon UH, Hahn JH, Koo BS, Lee GB, Kim H, Park HS, Yoon KO, Kim JH, Jung CH, Koh NH, Seo JS, Go SJ., Nucleic Acids Res. 33(2), 2005
PMID: 15673718
Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence.
Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Buttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klosgen U, Patschkowski T, Ruckert C, Rupp O, Schneiker S, Schuster SC, Vorholter FJ, Weber E, Puhler A, Bonas U, Bartels D, Kaiser O., J. Bacteriol. 187(21), 2005
PMID: 16237009
The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis
Vorhölter FJ, Schneiker S, Goesmann A, Krause L, Bekel T, Kaiser O, Linke B, Patschkowski T, Rückert C, Schmid J, Sidhu VK, Sieber V, Tauch A, Watt SA, Weisshaar B, Becker A, Niehaus K, Pühler A., 0
Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris.
Qian W, Jia Y, Ren SX, He YQ, Feng JX, Lu LF, Sun Q, Ying G, Tang DJ, Tang H, Wu W, Hao P, Wang L, Jiang BL, Zeng S, Gu WY, Lu G, Rong L, Tian Y, Yao Z, Fu G, Chen B, Fang R, Qiang B, Chen Z, Zhao GP, Tang JL, He C., Genome Res. 15(6), 2005
PMID: 15899963
Molecular secrets of bacterial type III effector proteins.
Lahaye T, Bonas U., Trends Plant Sci. 6(10), 2001
PMID: 11590067
Regulation of type III secretion systems.
Francis MS, Wolf-Watz H, Forsberg A., Curr. Opin. Microbiol. 5(2), 2002
PMID: 11934613
Port of entry--the type III secretion translocon.
Buttner D, Bonas U., Trends Microbiol. 10(4), 2002
PMID: 11912026
Functional analysis of HrpF, a putative type III translocon protein from Xanthomonas campestris pv. vesicatoria.
Buttner D, Nennstiel D, Klusener B, Bonas U., J. Bacteriol. 184(9), 2002
PMID: 11948151
Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100.
Watt SA, Wilke A, Patschkowski T, Niehaus K., Proteomics 5(1), 2005
PMID: 15619296
Esterase EstE from Xanthomonas vesicatoria ( Xv_EstE) is an outer membrane protein capable of hydrolyzing long-chain polar esters.
Talker-Huiber D, Jose J, Glieder A, Pressnig M, Stubenrauch G, Schwab H., Appl. Microbiol. Biotechnol. 61(5-6), 2003
PMID: 12764562
Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin.
Wai SN, Lindmark B, Soderblom T, Takade A, Westermark M, Oscarsson J, Jass J, Richter-Dahlfors A, Mizunoe Y, Uhlin BE., Cell 115(1), 2003
PMID: 14532000
Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae.
Dorward DW, Garon CF, Judd RC., J. Bacteriol. 171(5), 1989
PMID: 2496108
Untersuchungen zur Beteiligung des Lipopolysaccharids des phytopathogenen Bakteriums pv. in der Elicitierung von Pflanzenabwehr am Beispiel des in Zellkulturen des Nichtwirts Tabak ()
Meyer A., 2001
Gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria.
Li Z, Clarke AJ, Beveridge TJ., J. Bacteriol. 180(20), 1998
PMID: 9765585
Potential role of molecular mimicry between Helicobacter pylori lipopolysaccharide and host Lewis blood group antigens in autoimmunity.
Appelmelk BJ, Simoons-Smit I, Negrini R, Moran AP, Aspinall GO, Forte JG, De Vries T, Quan H, Verboom T, Maaskant JJ, Ghiara P, Kuipers EJ, Bloemena E, Tadema TM, Townsend RR, Tyagarajan K, Crothers JM Jr, Monteiro MA, Savio A, De Graaff J., Infect. Immun. 64(6), 1996
PMID: 8675304
Molecular signals required for type III secretion and translocation of the Xanthomonas campestris AvrBs2 protein to pepper plants.
Mudgett MB, Chesnokova O, Dahlbeck D, Clark ET, Rossier O, Bonas U, Staskawicz BJ., Proc. Natl. Acad. Sci. U.S.A. 97(24), 2000
PMID: 11078519
Direct biochemical evidence for type III secretion-dependent translocation of the AvrBs2 effector protein into plant cells.
Casper-Lindley C, Dahlbeck D, Clark ET, Staskawicz BJ., Proc. Natl. Acad. Sci. U.S.A. 99(12), 2002
PMID: 12060777
A hidden Markov model for predicting transmembrane helices in protein sequences
Glasgow J, Sonnhammer EL, von G, Krogh A., 1998
The release of outer membrane vesicles from the strains of enterotoxigenic Escherichia coli.
Wai SN, Takade A, Amako K., Microbiol. Immunol. 39(7), 1995
PMID: 8569529
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
Nielsen H, Engelbrecht J, Brunak S, von Heijne G., Protein Eng. 10(1), 1997
PMID: 9051728
Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy.
Mazia D, Schatten G, Sale W., J. Cell Biol. 66(1), 1975
PMID: 1095595
Proteome analysis of matrix vesicles isolated from femurs of chicken embryo.
Balcerzak M, Malinowska A, Thouverey C, Sekrecka A, Dadlez M, Buchet R, Pikula S., Proteomics 8(1), 2008
PMID: 18095356
Proteomic identification of endothelial proteins isolated in situ from atherosclerotic aorta via systemic perfusion.
Wu J, Liu W, Sousa E, Qiu Y, Pittman DD, Maganti V, Feldman J, Gill D, Lu Z, Dorner AJ, Schaub R, Tan XY., J. Proteome Res. 6(12), 2007
PMID: 17997517
WM Keck Foundation Biotechnology ResourceLaboratory
AUTHOR UNKNOWN, 0
Swiss-Prot Protein knowledgebase
AUTHOR UNKNOWN, 0
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 18518965
PubMed | Europe PMC

Suchen in

Google Scholar