Genetic tools and techniques for Chlamydomonas reinhardtii

Mussgnug JH (2015)
Applied Microbiology and Biotechnology 99(13): 5407-5418.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
URN
urn:nbn:de:0070-pub-27531659
Autor*in
Mussgnug, Jan H.UniBi
Einrichtung
Centrum für Biotechnologie > Institut für Biochemie und Biotechnik
Centrum für Biotechnologie > Arbeitsgruppe O. Kruse
Fakultät für Biologie > Algenbiotechnologie
Abstract / Bemerkung
The development of tools has always been a major driving force for the advancement of science. Optical microscopes were the first instruments that allowed discovery and descriptive studies of the subcellular features of microorganisms. Although optical and electron microscopes remained at the forefront of microbiological research tools since their inventions, the advent of molecular genetics brought about questions which had to be addressed with new Bgenetic tools^. The unicellular green microalgal genus Chlamydomonas, especially the most prominent species C. reinhardtii, has become a frequently used model organism for many diverse fields of research and molecular genetic analyses of C. reinhardtii, as well as the available genetic tools and techniques, have become increasingly sophisticated throughout the last decades. The aim of this review is to provide an overview of the molecular key features of C. reinhardtii and summarize the progress related to the development of tools and techniques for genetic engineering of this organism, from pioneering DNA transformation experiments to state-of-theart techniques for targeted nuclear genome editing and highthroughput screening approaches.
Stichworte
Chlamydomonas reinhardtiiGenetic engineeringGene silencingMolecular toolkitPromotersSelectable markers
Erscheinungsjahr
2015
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
99
Ausgabe
13
Seite(n)
5407-5418
Urheberrecht / Lizenzen
Creative Commons Namensnennung 3.0 Unported (CC BY 3.0)
ISSN
0175-7598
Page URI
https://pub.uni-bielefeld.de/record/2753165

Zitieren

Mussgnug JH. Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2015;99(13):5407-5418.
Mussgnug, J. H. (2015). Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology, 99(13), 5407-5418. doi:10.1007/s00253-015-6698-7
Mussgnug, Jan H. 2015. “Genetic tools and techniques for Chlamydomonas reinhardtii”. Applied Microbiology and Biotechnology 99 (13): 5407-5418.
Mussgnug, J. H. (2015). Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology 99, 5407-5418.
Mussgnug, J.H., 2015. Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology, 99(13), p 5407-5418.
J.H. Mussgnug, “Genetic tools and techniques for Chlamydomonas reinhardtii”, Applied Microbiology and Biotechnology, vol. 99, 2015, pp. 5407-5418.
Mussgnug, J.H.: Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 99, 5407-5418 (2015).
Mussgnug, Jan H. “Genetic tools and techniques for Chlamydomonas reinhardtii”. Applied Microbiology and Biotechnology 99.13 (2015): 5407-5418.
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19 Zitationen in Europe PMC

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AUTHOR UNKNOWN, 0
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