Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging.
Schäfermann J, Kliewer G, Losch J, Bednarz H, Giampa M, Niehaus K (2020)
Journal of mass spectrometry 55(3): e4488.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Schäfermann, Johanna;
Kliewer, Georg;
Losch, Jan;
Bednarz, HannaUniBi;
Giampa, Marco;
Niehaus, KarstenUniBi
Abstract / Bemerkung
Automated matrix deposition for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is crucial for producing reproducible analyte ion signals. Here we report an innovative method employing an automated immersion apparatus, which enables a robust matrix deposition within 5 minutes and with scalable throughput by using MAPS matrix and non-polar solvents. MSI results received from mouse heart and rat brain tissues were qualitatively similar to those from nozzle sprayed samples with respect to peak number and quality of the ion images. Overall, the immersion-method enables a fast and careful matrix deposition and has the future potential for implementation in clinical tissue diagnostics. This article is protected by copyright. All rights reserved.
Erscheinungsjahr
2020
Zeitschriftentitel
Journal of mass spectrometry
Band
55
Ausgabe
3
Art.-Nr.
e4488
Urheberrecht / Lizenzen
ISSN
1076-5174
eISSN
1096-9888
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld im Rahmen des DEAL-Vertrags gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2939578
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Schäfermann J, Kliewer G, Losch J, Bednarz H, Giampa M, Niehaus K. Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging. Journal of mass spectrometry. 2020;55(3): e4488.
Schäfermann, J., Kliewer, G., Losch, J., Bednarz, H., Giampa, M., & Niehaus, K. (2020). Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging. Journal of mass spectrometry, 55(3), e4488. doi:10.1002/jms.4488
Schäfermann, Johanna, Kliewer, Georg, Losch, Jan, Bednarz, Hanna, Giampa, Marco, and Niehaus, Karsten. 2020. “Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging.”. Journal of mass spectrometry 55 (3): e4488.
Schäfermann, J., Kliewer, G., Losch, J., Bednarz, H., Giampa, M., and Niehaus, K. (2020). Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging. Journal of mass spectrometry 55:e4488.
Schäfermann, J., et al., 2020. Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging. Journal of mass spectrometry, 55(3): e4488.
J. Schäfermann, et al., “Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging.”, Journal of mass spectrometry, vol. 55, 2020, : e4488.
Schäfermann, J., Kliewer, G., Losch, J., Bednarz, H., Giampa, M., Niehaus, K.: Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging. Journal of mass spectrometry. 55, : e4488 (2020).
Schäfermann, Johanna, Kliewer, Georg, Losch, Jan, Bednarz, Hanna, Giampa, Marco, and Niehaus, Karsten. “Immersion by Rotation-Based Application of the Matrix for Fast and Reproducible Sample Preparations and Robust Results in Mass Spectrometry Imaging.”. Journal of mass spectrometry 55.3 (2020): e4488.
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