Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>

Schilbert H, Pucker B, Ries D, Viehöver P, Micic Z, Dreyer F, Beckmann K, Wittkop B, Weisshaar B, Holtgräwe D (2022)
Genes 13(7): 1131.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Schilbert, HannaUniBi; Pucker, Boas; Ries, David; Viehöver, PriscaUniBi; Micic, Zeljko; Dreyer, Felix; Beckmann, Katrin; Wittkop, Benjamin; Weisshaar, BerndUniBi ; Holtgräwe, DanielaUniBi
Abstract / Bemerkung
Rapeseed (Brassica napusL.) is an important oil crop and harbours the potential to serve as a highly productive source of protein. This protein exhibits an excellent amino acid composition and has a high nutritional value for humans. Seed protein content (SPC) and seed oil content (SOC) are two complex quantitative and polygenic traits which are negatively correlated and assumed to be controlled by additive and epistatic effects. A reduction of seed glucosinolate (GSL) content is desired as GSLs cause a stringent and bitter taste. The goal here was the identification of genomic intervals relevant for seed GSL content and SPC/SOC. Mapping-by-sequencing (MBS) revealed 30 and 15 new and known genomic intervals associated with seed GSL content and SPC/SOC, respectively. Within these intervals we identified known but also so far unknown putatively causal genes and sequence variants. A 4 bp insertion in theMYB28homolog on C09 shows a significant correlation with a reduction in seed GSL content. This study provides insights into the genetic architecture and potential mechanisms underlying seed quality traits, which will enhance future breeding approaches inB. napus.
Stichworte
breeding; bulk segregant analysis; delta allele frequencies; glucosinolates; gene expression; interval detection; MYB; rapeseed; seed protein content; seed oil content
Erscheinungsjahr
2022
Zeitschriftentitel
Genes
Band
13
Ausgabe
7
Art.-Nr.
1131
eISSN
2073-4425
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2963558

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Schilbert H, Pucker B, Ries D, et al. Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>. Genes. 2022;13(7): 1131.
Schilbert, H., Pucker, B., Ries, D., Viehöver, P., Micic, Z., Dreyer, F., Beckmann, K., et al. (2022). Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>. Genes, 13(7), 1131. https://doi.org/10.3390/genes13071131
Schilbert, Hanna, Pucker, Boas, Ries, David, Viehöver, Prisca, Micic, Zeljko, Dreyer, Felix, Beckmann, Katrin, Wittkop, Benjamin, Weisshaar, Bernd, and Holtgräwe, Daniela. 2022. “Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>”. Genes 13 (7): 1131.
Schilbert, H., Pucker, B., Ries, D., Viehöver, P., Micic, Z., Dreyer, F., Beckmann, K., Wittkop, B., Weisshaar, B., and Holtgräwe, D. (2022). Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>. Genes 13:1131.
Schilbert, H., et al., 2022. Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>. Genes, 13(7): 1131.
H. Schilbert, et al., “Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>”, Genes, vol. 13, 2022, : 1131.
Schilbert, H., Pucker, B., Ries, D., Viehöver, P., Micic, Z., Dreyer, F., Beckmann, K., Wittkop, B., Weisshaar, B., Holtgräwe, D.: Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>. Genes. 13, : 1131 (2022).
Schilbert, Hanna, Pucker, Boas, Ries, David, Viehöver, Prisca, Micic, Zeljko, Dreyer, Felix, Beckmann, Katrin, Wittkop, Benjamin, Weisshaar, Bernd, and Holtgräwe, Daniela. “Mapping-by-sequencing reveals genomic regions associated with seed quality parameters in <em>Brassica napus</em>”. Genes 13.7 (2022): 1131.
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2022-07-07T07:02:22Z
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Supplementary material related to MBS analysis of seed quality parameters in Brassica napus
Pucker B, Schilbert H, Viehöver P, Weisshaar B, Holtgräwe D (2022)
Bielefeld University.
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PMID: 35885914
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Preprint: 10.1101/2022.06.01.494149

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