- AutorIn
- Andreia de Albuquerque
- Ilja Kubisch
- Georg Breier
- Gudrun Stamminger
- Nikos Fersis
- Astrid Eichler
- Sepp Kaul
- Ulrich Stölzel
- Titel
- Multimarker Gene Analysis of Circulating Tumor Cells in Pancreatic Cancer Patients: A Feasibility Study
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-133530
- Quellenangabe
- Oncology 2012;82:3–10, ISSN: 0030-2414
- Erstveröffentlichung
- 2012
- Abstract (DE)
- Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
- Abstract (EN)
- Objective: The aim of this study was to develop an immunomagnetic/real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay and assess its clinical value for the molecular detection of circulating tumor cells (CTCs) in peripheral blood of pancreatic cancer patients. Methods: The presence of CTCs was evaluated in 34 pancreatic cancer patients before systemic therapy and in 40 healthy controls, through immunomagnetic enrichment, using the antibodies BM7 and VU1D9 [targeting mucin 1 and epithelial cell adhesion molecule (EpCAM), respectively], followed by real-time RT-PCR analysis of the genes KRT19, MUC1, EPCAM, CEACAM5 and BIRC5. Results: The developed assay showed high specificity, as none of the healthy controls were found to be positive for the multimarker gene panel. CTCs were detected in 47.1% of the pancreatic cancer patients before the beginning of systemic treatment. Shorter median progression-free survival (PFS) was observed for patients who had at least one detectable tumor-associated transcript, compared with patients who were CTC negative. Median PFS time was 66.0 days [95% confidence interval (CI) 44.8–87.2] for patients with baseline CTC positivity and 138.0 days (95% CI 124.1–151.9) for CTC-negative patients (p = 0.01, log-rank test). Conclusion: Our results suggest that in addition to the current prognostic methods, CTC analysis represents a potential complementary tool for prediction of outcome in pancreatic cancer patients.
- Andere Ausgabe
- DOI: 10.1159/000335479
- Volltext des Artikels, der zuerst in der Zeitschrift "Oncology" des Karger-Verlages erschienen ist.
Link: http://dx.doi.org/10.1159/000335479 - Freie Schlagwörter (DE)
- zirkulierende Tumorzellen, Bauchspeicheldrüsenkrebs, Reverse Transkriptase-Polymerase-Ketten-Reaktion
- Freie Schlagwörter (EN)
- Circulating tumor cells, CTC, Pancreatic cancer, Reverse transcriptase real-time polymerase chain reaction, RT-PCR
- Klassifikation (DDC)
- 610
- Klassifikation (RVK)
- XA 10000
- Verlag
- Karger, Basel
- Förder- / Projektangaben
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa-133530
- Veröffentlichungsdatum Qucosa
- 12.02.2014
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis