- AutorIn
- Stefan Propping
- Manja Newe
- Kristina Lorenz
- Manfred P. Wirth
- Ursula Ravens
- Titel
- β-Adrenoceptor-Mediated Relaxation of Carbachol-Pre-Contracted Mouse Detrusor
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-203472
- Quellenangabe
- Urologia internationalis Vol.95 (2015) No.1:92-98 ISSN: 1423-0399
- Erstveröffentlichung
- 2015
- Abstract (DE)
- Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
- Abstract (EN)
- Aims: To study the β-adrenoceptor subtypes involved in the relaxation responses to (-)-isoprenaline in carbachol-pre-contracted (CCh) mouse detrusor muscle with intact and denuded mucosa. Methods: Isolated muscle strips from the urinary bladder of male C57BL6 mice or β2-adrenoceptor knockout mice were pre-contracted with CCh, 1 µM and relaxed with increasing concentrations of the β-adrenoceptor (β-AR) agonist (-)-isoprenaline and forskolin. For estimating the β-AR subtypes involved, subtype-selective receptor blockers were used, that is, CGP 20712A (β1-ARs), ICI 118,551 (β2-ARs), and L748,337 (β3-ARs). Results: Unlike in KCl-pre-contracted muscle, the mucosa did not affect the sensitivity of the relaxation response to (-)-isoprenaline in CCh-pre-contracted murine detrusor strips. Increasing concentrations of (-)-isoprenaline produced a biphasic concentration-relaxation response without any difference both during the presence and absence of mucosa. The relaxation fraction produced by low (-)-isoprenaline concentrations was mediated by β2-AR as evidenced by a shift of the concentration-response curve to higher concentrations with ICI 118,551, but not with CGP 20712A and L748,337, and by the absence of this fraction in β2-AR-KO mice. The relaxation response with low sensitivity to (-)-isoprenaline was not affected by any of the β-AR subtype-selective blockers and was the only response detected in detrusor strips from β2-AR-KO mice. Conclusions: In CCh-pre-contracted mouse detrusor, β2-ARs are responsible for the relaxation component with high sensitivity to (-)-isoprenaline as indicated by the conversion of a biphasic into a monophasic CRC with ICI 118,551 or by its absence in β2-AR KO mice. The mucosa does not impair relaxation under these conditions.
- Andere Ausgabe
- DOI: 10.1159/000369075
- Volltext des Artikels, der zuerst in der Zeitschrift "Urologia Internationalis" des Karger-Verlages erschienen ist.
Link: https://dx.doi.org/10.1159/000369075 - Freie Schlagwörter (DE)
- Muskulatur, Entspannung, Mukosa
- Freie Schlagwörter (EN)
- Detrusor smooth muscle, Carbachol-induced contractions, Relaxation, Mucosa, β-Adrenoceptors
- Klassifikation (DDC)
- 570, 610
- Klassifikation (RVK)
- WA 15000, XA 10000
- Verlag
- Karger, Basel
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa-203472
- Veröffentlichungsdatum Qucosa
- 08.06.2016
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis