- AutorIn
- Susanne Melzer
- Christian Sonnendecker
- Christina Föllner
- Wolfgang Zimmermann
- Titel
- Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.
- Untertitel
- Stepwise error-prone PCR and DNA shuffling changed the pH activityrange and product specificity of the cyclodextrin glucanotransferasefrom an alkaliphilic Bacillus sp.
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa-172353
- Quellenangabe
- FEBS Open Bio 2015, 5, S. 528–534
- Erstveröffentlichung
- 2015
- Abstract (EN)
- Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilic Bacillus sp. G-825-6 converts starch mainly to c-cyclodextrin (CD8). A combination of error-prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD8 and a broad pH activity range. The variant S54 with seven amino acid substitutions showed a 1.2-fold increase in CD8-synthesizing activity and the product ratio of CD7:CD8 was shifted to 1:7 compared to 1:3 of the wild-type enzyme. Nine amino acid substitutions of the cyclodextrin glucanotransferase were performed to generate the variant S35 active in a pH range 4.0–10.0. Compared to the wild-type enzyme which is inactive below pH 6.0, S35 retained 70% of its CD8-synthesizing activity at pH 4.0.
- Andere Ausgabe
- Link zur Originalpublikation in der Zeitschrift FEBS Open Bio
Link: http://dx.doi.org/10.1016/j.fob.2015.06.002 - Freie Schlagwörter (DE)
- Cyclodextrin-Glucosyl-Transferase, Bacillus sp., Gamma-Cyclodextrin, zufällige Mutagenese, DNA-Shuffling
- Freie Schlagwörter (EN)
- Cyclodextrin glucanotransferase, Bacillus sp., Gamma-cyclodextrin, Random mutagenesis, DNA shuffling
- Klassifikation (DDC)
- 572
- Herausgeber (Institution)
- Universität Leipzig
- Verlag
- Elsevier, Cambridge
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa-172353
- Veröffentlichungsdatum Qucosa
- 29.06.2015
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch