- AutorIn
- Simone Prömel
- Franziska Fiedler
- Claudia Binder
- Jana Winkler
- Torsten Schöneberg
- Doreen Thor
- Titel
- Deciphering and modulating G protein signalling in C. elegans using the DREADD technology
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa-207728
- Quellenangabe
- Scientific reports 6 (2016):28901 Doi: 10.1038/srep28901
- Erstveröffentlichung
- 2016
- Abstract (EN)
- G-protein signalling is an evolutionary conserved concept highlighting its fundamental impact on developmental and functional processes. Studies on the effects of G protein signals on tissues as well as an entire organism are often conducted in Caenorhabditis elegans. To understand and control dynamics and kinetics of the processes involved, pharmacological modulation of specific G protein pathways would be advantageous, but is difficult due to a lack in accessibility and regulation. To provide this option, we designed G protein-coupled receptor-based designer receptors (DREADDs) for C. elegans. Initially described in mammalian systems, these modified muscarinic acetylcholine receptors are activated by the inert drug clozapine N-oxide, but not by their endogenous agonists. We report a novel C. elegans-specific DREADD, functionally expressed and specifically activating Gq-protein signalling in vitro and in vivo which we used for modulating mating behaviour. Therefore, this novel designer receptor demonstrates the possibility to pharmacologically control physiological functions in C. elegans.
- Andere Ausgabe
- Link zur Originalpublikation in der Zeitschrift Scientific reports
Link: http://dx.doi.org/10.1038/srep28901 - Freie Schlagwörter (DE)
- DREADD, G-Protein, Caenorhabditis elegans
- Freie Schlagwörter (EN)
- Designer receptors exclusively activated by designer drugs (DREADD), G-protein, Caenorhabditis elegans
- Klassifikation (DDC)
- 572
- Herausgeber (Institution)
- Universität Leipzig
- Verlag
- Nature Publ., London
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa-207728
- Veröffentlichungsdatum Qucosa
- 28.07.2016
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch