- AutorIn
- Christoph Ottenheim
- Carl Verdejo
- Wolfgang Zimmermann
- Jin Chuan Wu
- Titel
- Hemicellulase production by Aspergillus niger DSM 26641 in hydrothermal palm oil empty fruit bunch hydrolysate and transcriptome analysis
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-168534
- Quellenangabe
- Journal of Bioscience and Bioengineering
Erscheinungsjahr: 2014
Jahrgang: 118
Heft: 6
Seiten: 696-701
DOI: 10.1016/j.jbiosc.2014.05.014 - Erstveröffentlichung
- 2014
- Abstract (EN)
- Palm oil empty fruit bunches (EFB) is an abundant and cheap lignocellulose material in South East Asia. Its use as the sole medium for producing lignocellulose-hydrolyzing enzymes would increase its commercial value. A newly isolated Aspergillus niger DSM 26641 was investigated for its capability of producing hemicellulases in EFB hydrolysate obtained by treatment with pressurized hot water (1-20%, w/v) at 120-180◦C in a 1 L Parr reactor for 10-60 min. The optimal hydrolysate for the fungal growth and endoxylanase production was obtained when 10% (w/v) of empty fruit bunch was treated at 120◦C or 150◦C for 10 min, giving an endoxylanase activity of 24.5 mU ml-1 on RBB-Xylan and a saccharification activity of 5 U ml-1 on xylan (DNS assay). When the hydrolysates were produced at higher temperatures, longer treatment times or higher biomass contents, only less than 20% of the above maximal endoxylanase activity was detected, possibly due to the higher carbohydrate concentrations in the medium. Transcriptome analysis showed that 3 endoxylanases (expression levels 59-100%, the highest level was set as 100%), 2 b-xylosidases (4%), 4 side chain-cleaving arabinofuranosidases (1-95%), 1 acetyl xylan esterase (9%) and 2 ferulic acid esterases (0.3-9%) were produced together.
- Andere Ausgabe
- Hemicellulase production by Aspergillus niger DSM 26641 in hydrothermal palm oil empty fruit bunch hydrolysate and transcriptome analysis
DOI: 10.1016/j.jbiosc.2014.05.014 - Freie Schlagwörter (EN)
- Filamentous Fungi, Submerged culture, Empty fruit bunch, Hydrolysate, Hydrothermal treatment, Endoxylanase, Hemicellulase
- Klassifikation (DDC)
- 572
- Herausgeber (Institution)
- Universität Leipzig
- Version / Begutachtungsstatus
- angenommene Version / Postprint / Autorenversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-168534
- Veröffentlichungsdatum Qucosa
- 01.12.2017
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch