@article{Baschieri2014Spati-29329,
  year={2014},
  doi={10.1038/ncomms5839},
  title={Spatial control of Cdc42 signalling by a GM130-RasGRF complex regulates polarity and tumorigenesis},
  volume={5},
  issn={0256-7865},
  journal={Nature communications},
  author={Baschieri, Francesco and Confalonieri, Stefano and Bertalot, Giovanni and Di Fiore, Pier Paolo and Dietmaier, Wolfgang and Leist, Marcel and Crespo, Piero and Macara, Ian G. and Farhan, Hesso},
  note={Article Number: 4839}
}

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29329">
    <dc:contributor>Farhan, Hesso</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <dc:creator>Leist, Marcel</dc:creator>
    <dc:contributor>Confalonieri, Stefano</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Baschieri, Francesco</dc:contributor>
    <dcterms:title>Spatial control of Cdc42 signalling by a GM130-RasGRF complex regulates polarity and tumorigenesis</dcterms:title>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/29329/1/Leist_0-258846.pdf"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/29329"/>
    <dc:creator>Baschieri, Francesco</dc:creator>
    <dc:creator>Macara, Ian G.</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Crespo, Piero</dc:creator>
    <dc:contributor>Bertalot, Giovanni</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-11-27T10:00:56Z</dc:date>
    <dcterms:abstract xml:lang="eng">The small GTPase ​Cdc42 is a key regulator of polarity, but little is known in mammals about its spatial regulation and the relevance of spatial ​Cdc42 pools for polarity. Here we report the identification of a ​GM130–RasGRF complex as a regulator of ​Cdc42 at the Golgi. Silencing ​GM130 results in RasGRF-dependent inhibition of the Golgi pool of ​Cdc42, but does not affect ​Cdc42 at the cell surface. Furthermore, active ​Cdc42 at the Golgi is important to sustain asymmetric front–rear ​Cdc42-GTP distribution in directionally migrating cells. Concurrent to ​Cdc42 inhibition, silencing ​GM130 also results in RasGRF-dependent Ras-ERK pathway activation. Moreover, depletion of ​GM130 is sufficient to induce ​E-cadherin downregulation, indicative of a loss in cell polarity and epithelial identity. Accordingly, ​GM130 expression is frequently lost in colorectal and breast cancer patients. These findings establish a previously unrecognized role for a ​GM130–RasGRF–​Cdc42 connection in regulating polarity and tumorigenesis.</dcterms:abstract>
    <dc:contributor>Dietmaier, Wolfgang</dc:contributor>
    <dc:contributor>Di Fiore, Pier Paolo</dc:contributor>
    <dc:creator>Farhan, Hesso</dc:creator>
    <dc:contributor>Macara, Ian G.</dc:contributor>
    <dc:creator>Dietmaier, Wolfgang</dc:creator>
    <dc:contributor>Crespo, Piero</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-11-27T10:00:56Z</dcterms:available>
    <dc:creator>Confalonieri, Stefano</dc:creator>
    <dcterms:issued>2014</dcterms:issued>
    <dc:creator>Di Fiore, Pier Paolo</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/29329/1/Leist_0-258846.pdf"/>
    <dc:creator>Bertalot, Giovanni</dc:creator>
    <dc:contributor>Leist, Marcel</dc:contributor>
  </rdf:Description>
</rdf:RDF>