Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways

Ravid, Tommer; Kreft, Stefan G.; Hochstrasser, Mark

Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways

Dateien

Ravid_0-269548.pdfGröße: 445.31 KBDownloads: 206

Datum

2006

Autor:innen

Ravid, Tommer

Kreft, Stefan G.

Hochstrasser, Mark

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

The EMBO journal. 2006, 25(3), pp. 533-543. ISSN 0261-4189. eISSN 1460-2075. Available under: doi: 10.1038/sj.emboj.7600946

Zusammenfassung

The yeast Doa10 ubiquitin (Ub) ligase resides in the endoplasmic reticulum (ER)/nuclear envelope (NE), where it functions in ER-associated degradation (ERAD). Doa10 substrates include non-ER proteins such as the transcription factor Mat alpha2. Here, we expand the range of Doa10 substrates to include a defective kinetochore component, a mutant NE membrane protein, and a substrate-regulated human ER enzyme. For all these substrates, Doa10 requires two Ub-conjugating enzymes, Ubc6 and Ubc7, as well as the Ubc7 cofactor Cue1. Based on a novel genomic screen of a comprehensive gene deletion library and other data, these four proteins appear to be the only nonessential and nonredundant factors generally required for Doa10-mediated ubiquitination. Notably, the Cdc48 ATPase facilitates degradation of membrane-embedded Doa10 substrates, but is not required for any tested soluble Doa10 substrates. This distinction is maintained even when comparing membrane and soluble proteins bearing the same degradation signal. Thus, while Doa10 ubiquitinates both membrane and soluble proteins, the mechanisms of subsequent proteasome targeting differ.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

RAVID, Tommer, Stefan G. KREFT, Mark HOCHSTRASSER, 2006. Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways. In: The EMBO journal. 2006, 25(3), pp. 533-543. ISSN 0261-4189. eISSN 1460-2075. Available under: doi: 10.1038/sj.emboj.7600946

BibTex

@article{Ravid2006Membr-29524,
  year={2006},
  doi={10.1038/sj.emboj.7600946},
  title={Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways},
  number={3},
  volume={25},
  issn={0261-4189},
  journal={The EMBO journal},
  pages={533--543},
  author={Ravid, Tommer and Kreft, Stefan G. and Hochstrasser, Mark}
}

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