Phylogenetic and environmental diversity of DsrAB-type dissimilatory (bi)sulfite reductases

Müller, Albert Leopold; Kjeldsen, Kasper Urup; Rattei, Thomas; Pester, Michael; Loy, Alexander

Phylogenetic and environmental diversity of DsrAB-type dissimilatory (bi)sulfite reductases

Dateien

Mueller_0-269844.pdfGröße: 385.86 KBDownloads: 600

Datum

2015

Autor:innen

Müller, Albert Leopold

Kjeldsen, Kasper Urup

Rattei, Thomas

Pester, Michael

Loy, Alexander

Open Access-Veröffentlichung

Open Access Hybrid

Sammlungen

Biologie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

The ISME Journal. 2015, 9(5), pp. 1152-1165. ISSN 1751-7362. eISSN 1751-7370. Available under: doi: 10.1038/ismej.2014.208

Zusammenfassung

The energy metabolism of essential microbial guilds in the biogeochemical sulfur cycle is based on a DsrAB-type dissimilatory (bi)sulfite reductase that either catalyzes the reduction of sulfite to sulfide during anaerobic respiration of sulfate, sulfite and organosulfonates, or acts in reverse during sulfur oxidation. Common use of dsrAB as a functional marker showed that dsrAB richness in many environments is dominated by novel sequence variants and collectively represents an extensive, largely uncharted sequence assemblage. Here, we established a comprehensive, manually curated dsrAB/DsrAB database and used it to categorize the known dsrAB diversity, reanalyze the evolutionary history of dsrAB and evaluate the coverage of published dsrAB-targeted primers. Based on a DsrAB consensus phylogeny, we introduce an operational classification system for environmental dsrAB sequences that integrates established taxonomic groups with operational taxonomic units (OTUs) at multiple phylogenetic levels, ranging from DsrAB enzyme families that reflect reductive or oxidative DsrAB types of bacterial or archaeal origin, superclusters, uncultured family-level lineages to species-level OTUs. Environmental dsrAB sequences constituted at least 13 stable family-level lineages without any cultivated representatives, suggesting that major taxa of sulfite/sulfate-reducing microorganisms have not yet been identified. Three of these uncultured lineages occur mainly in marine environments, while specific habitat preferences are not evident for members of the other 10 uncultured lineages. In summary, our publically available dsrAB/DsrAB database, the phylogenetic framework, the multilevel classification system and a set of recommended primers provide a necessary foundation for large-scale dsrAB ecology studies with next-generation sequencing methods.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

MÜLLER, Albert Leopold, Kasper Urup KJELDSEN, Thomas RATTEI, Michael PESTER, Alexander LOY, 2015. Phylogenetic and environmental diversity of DsrAB-type dissimilatory (bi)sulfite reductases. In: The ISME Journal. 2015, 9(5), pp. 1152-1165. ISSN 1751-7362. eISSN 1751-7370. Available under: doi: 10.1038/ismej.2014.208

BibTex

@article{Muller2015-05Phylo-30372,
  year={2015},
  doi={10.1038/ismej.2014.208},
  title={Phylogenetic and environmental diversity of DsrAB-type dissimilatory (bi)sulfite reductases},
  number={5},
  volume={9},
  issn={1751-7362},
  journal={The ISME Journal},
  pages={1152--1165},
  author={Müller, Albert Leopold and Kjeldsen, Kasper Urup and Rattei, Thomas and Pester, Michael and Loy, Alexander}
}

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