The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum

Gamerdinger, Martin; Hanebuth, Marie A.; Frickey, Tancred; Deuerling, Elke

The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum

Dateien

Gamerdinger_0-291098.pdfGröße: 2.09 MBDownloads: 693

Datum

2015

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Science. 2015, 348(6231), pp. 201-207. ISSN 0036-8075. eISSN 1095-9203. Available under: doi: 10.1126/science.aaa5335

Zusammenfassung

The sorting of proteins to the appropriate compartment is one of the most fundamental cellular processes. We found that in the model organism Caenorhabditis elegans, correct cotranslational endoplasmic reticulum (ER) transport required the suppressor activity of the nascent polypeptide-associated complex (NAC). NAC did not affect the accurate targeting of ribosomes to ER translocons mediated by the signal recognition particle (SRP) pathway but inhibited additional unspecific contacts between ribosomes and translocons by blocking their autonomous binding affinity. NAC depletion shortened the life span of Caenorhabditis elegans, caused global mistargeting of translating ribosomes to the ER, and provoked incorrect import of mitochondrial proteins into the ER lumen, resulting in a strong impairment of protein homeostasis in both compartments. Thus, the antagonistic targeting activity of NAC is important in vivo to preserve the robustness and specificity of cellular protein-sorting routes.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

GAMERDINGER, Martin, Marie A. HANEBUTH, Tancred FRICKEY, Elke DEUERLING, 2015. The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum. In: Science. 2015, 348(6231), pp. 201-207. ISSN 0036-8075. eISSN 1095-9203. Available under: doi: 10.1126/science.aaa5335

BibTex

@article{Gamerdinger2015princ-31204,
  year={2015},
  doi={10.1126/science.aaa5335},
  title={The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum},
  number={6231},
  volume={348},
  issn={0036-8075},
  journal={Science},
  pages={201--207},
  author={Gamerdinger, Martin and Hanebuth, Marie A. and Frickey, Tancred and Deuerling, Elke}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/31204">
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Hanebuth, Marie A.</dc:contributor>
    <dcterms:title>The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-06-19T06:12:45Z</dc:date>
    <dc:contributor>Gamerdinger, Martin</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Deuerling, Elke</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/31204/1/Gamerdinger_0-291098.pdf"/>
    <dc:language>eng</dc:language>
    <dc:creator>Deuerling, Elke</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Frickey, Tancred</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-06-19T06:12:45Z</dcterms:available>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/31204"/>
    <dcterms:abstract xml:lang="eng">The sorting of proteins to the appropriate compartment is one of the most fundamental cellular processes. We found that in the model organism Caenorhabditis elegans, correct cotranslational endoplasmic reticulum (ER) transport required the suppressor activity of the nascent polypeptide-associated complex (NAC). NAC did not affect the accurate targeting of ribosomes to ER translocons mediated by the signal recognition particle (SRP) pathway but inhibited additional unspecific contacts between ribosomes and translocons by blocking their autonomous binding affinity. NAC depletion shortened the life span of Caenorhabditis elegans, caused global mistargeting of translating ribosomes to the ER, and provoked incorrect import of mitochondrial proteins into the ER lumen, resulting in a strong impairment of protein homeostasis in both compartments. Thus, the antagonistic targeting activity of NAC is important in vivo to preserve the robustness and specificity of cellular protein-sorting routes.</dcterms:abstract>
    <dcterms:issued>2015</dcterms:issued>
    <dc:creator>Hanebuth, Marie A.</dc:creator>
    <dc:contributor>Frickey, Tancred</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Gamerdinger, Martin</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/31204/1/Gamerdinger_0-291098.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja