Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1

Felux, Ann-Katrin; Spiteller, Dieter; Klebensberger, Janosch; Schleheck, David

Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1

Dateien

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Datum

2015

Autor:innen

Felux, Ann-Katrin

Spiteller, Dieter

Klebensberger, Janosch

Schleheck, David

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Proceedings of the National Academy of Sciences of the United States of America : PNAS. 2015, 112(31), pp. E4298-E4305. ISSN 0027-8424. eISSN 1091-6490. Available under: doi: 10.1073/pnas.1507049112

Zusammenfassung

Sulfoquinovose (SQ; 6-deoxy-6-sulfoglucose) is the polar head group of the plant sulfolipid SQ-diacylglycerol, and SQ comprises a major proportion of the organosulfur in nature, where it is degraded by bacteria. A first degradation pathway for SQ has been demonstrated recently, a "sulfoglycolytic" pathway, in addition to the classical glycolytic (Embden-Meyerhof) pathway in Escherichia coli K-12; half of the carbon of SQ is abstracted as dihydroxyacetonephosphate (DHAP) and used for growth, whereas a C3-organosulfonate, 2,3-dihydroxypropane sulfonate (DHPS), is excreted. The environmental isolate Pseudomonas putida SQ1 is also able to use SQ for growth, and excretes a different C3-organosulfonate, 3-sulfolactate (SL). In this study, we revealed the catabolic pathway for SQ in P. putida SQ1 through differential proteomics and transcriptional analyses, by in vitro reconstitution of the complete pathway by five heterologously produced enzymes, and by identification of all four organosulfonate intermediates. The pathway follows a reaction sequence analogous to the Entner-Doudoroff pathway for glucose-6-phosphate: It involves an NAD(+)-dependent SQ dehydrogenase, 6-deoxy-6-sulfogluconolactone (SGL) lactonase, 6-deoxy-6-sulfogluconate (SG) dehydratase, and 2-keto-3,6-dideoxy-6-sulfogluconate (KDSG) aldolase. The aldolase reaction yields pyruvate, which supports growth of P. putida, and 3-sulfolactaldehyde (SLA), which is oxidized to SL by an NAD(P)(+)-dependent SLA dehydrogenase. All five enzymes are encoded in a single gene cluster that includes, for example, genes for transport and regulation. Homologous gene clusters were found in genomes of other P. putida strains, in other gamma-Proteobacteria, and in beta- and alpha-Proteobacteria, for example, in genomes of Enterobacteria, Vibrio, and Halomonas species, and in typical soil bacteria, such as Burkholderia, Herbaspirillum, and Rhizobium.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

FELUX, Ann-Katrin, Dieter SPITELLER, Janosch KLEBENSBERGER, David SCHLEHECK, 2015. Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1. In: Proceedings of the National Academy of Sciences of the United States of America : PNAS. 2015, 112(31), pp. E4298-E4305. ISSN 0027-8424. eISSN 1091-6490. Available under: doi: 10.1073/pnas.1507049112

BibTex

@article{Felux2015-07-20Entne-31607,
  year={2015},
  doi={10.1073/pnas.1507049112},
  title={Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1},
  number={31},
  volume={112},
  issn={0027-8424},
  journal={Proceedings of the National Academy of Sciences of the United States of America : PNAS},
  pages={E4298--E4305},
  author={Felux, Ann-Katrin and Spiteller, Dieter and Klebensberger, Janosch and Schleheck, David}
}

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