Identification of the Gene Cluster for the Anaerobic Degradation of 3,5-Dihydroxybenzoate (α-Resorcylate) in Thauera aromatica Strain AR-1

Molina-Fuentes, Agueda; Pacheco, Daniel; Marin, Patricia; Philipp, Bodo; Schink, Bernhard; Marqués, Silvia

Identification of the Gene Cluster for the Anaerobic Degradation of 3,5-Dihydroxybenzoate (α-Resorcylate) in Thauera aromatica Strain AR-1

Dateien

Molina_0-309362.pdfGröße: 1.65 MBDownloads: 427

Datum

2015

Autor:innen

Molina-Fuentes, Agueda

Pacheco, Daniel

Marin, Patricia

Philipp, Bodo

Schink, Bernhard

Marqués, Silvia

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Applied and Environmental Microbiology. 2015, 81(20), pp. 7201-7214. ISSN 0003-6919. eISSN 1098-5336. Available under: doi: 10.1128/AEM.01698-15

Zusammenfassung

Thauera aromatica strain AR-1 degrades 3,5-dihydroxybenzoate (3,5-DHB) with nitrate as an electron acceptor. Previous biochemical studies have shown that this strain converts 3,5-DHB to hydroxyhydroquinone (1,2,4-trihydroxybenzene) through water-dependent hydroxylation of the aromatic ring and subsequent decarboxylation, and they suggest a pathway homologous to that described for the anaerobic degradation of 1,3-dihydroxybenzene (resorcinol) by Azoarcus anaerobius. Southern hybridization of a T. aromatica strain AR-1 gene library identified a 25-kb chromosome region based on its homology with A. anaerobius main pathway genes. Sequence analysis defined 20 open reading frames. Knockout mutations of the most relevant genes in the pathway were generated by reverse genetics. Physiological and biochemical analyses identified the genes for the three main steps in the pathway which were homologous to those described in A. anaerobius and suggested the function of several auxiliary genes possibly involved in enzyme maturation and intermediate stabilization. However, T. aromatica strain AR-1 had an additional enzyme to metabolize hydroxyhydroquinone, a putative cytoplasmic quinone oxidoreductase. In addition, a specific tripartite ATP-independent periplasmic (TRAP) transport system was required for efficient growth on 3,5-DHB. Reverse transcription-PCR (RT-PCR) analysis showed that the pathway genes were organized in five 3,5-DHB-inducible operons, three of which have been shown to be under the control of a single LysR-type transcriptional regulator, DbdR. Despite sequence homology, the genetic organizations of the clusters in T. aromatica strain AR-1 and A. anaerobius differed substantially.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

MOLINA-FUENTES, Agueda, Daniel PACHECO, Patricia MARIN, Bodo PHILIPP, Bernhard SCHINK, Silvia MARQUÉS, 2015. Identification of the Gene Cluster for the Anaerobic Degradation of 3,5-Dihydroxybenzoate (α-Resorcylate) in Thauera aromatica Strain AR-1. In: Applied and Environmental Microbiology. 2015, 81(20), pp. 7201-7214. ISSN 0003-6919. eISSN 1098-5336. Available under: doi: 10.1128/AEM.01698-15

BibTex

@article{MolinaFuentes2015Ident-32252,
  year={2015},
  doi={10.1128/AEM.01698-15},
  title={Identification of the Gene Cluster for the Anaerobic Degradation of 3,5-Dihydroxybenzoate (α-Resorcylate) in Thauera aromatica Strain AR-1},
  number={20},
  volume={81},
  issn={0003-6919},
  journal={Applied and Environmental Microbiology},
  pages={7201--7214},
  author={Molina-Fuentes, Agueda and Pacheco, Daniel and Marin, Patricia and Philipp, Bodo and Schink, Bernhard and Marqués, Silvia}
}

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