Molecular Evolution of the Neural Crest Regulatory Network in Ray-Finned Fish

Kratochwil, Claudius F.; Geißler, Laura; Irisarri, Iker; Meyer, Axel

Molecular Evolution of the Neural Crest Regulatory Network in Ray-Finned Fish

Dateien

Kratochwil_0-312895.pdfGröße: 1.12 MBDownloads: 245

Datum

2015

Angaben zur Forschungsförderung

European Union (EU): 293700

Projekt

Comparative genomics of parallel in repeated adaptive radiations (GenAdap)

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Genome Biology and Evolution. 2015, 7(11), pp. 3033-3046. eISSN 1759-6653. Available under: doi: 10.1093/gbe/evv200

Zusammenfassung

Gene regulatory networks (GRN) are central to developmental processes. They are composed of transcription factors and signaling molecules orchestrating gene expression modules that tightly regulate the development of organisms. The neural crest (NC) is a multipotent cell population that is considered a key innovation of vertebrates. Its derivatives contribute to shaping the astounding morphological diversity of jaws, teeth, head skeleton, or pigmentation. Here, we study the molecular evolution of the NC GRN by analyzing patterns of molecular divergence for a total of 36 genes in 16 species of bony fishes. Analyses of nonsynonymous to synonymous substitution rate ratios (dN/dS) support patterns of variable selective pressures among genes deployed at different stages of NC development, consistent with the developmental hourglass model. Model-based clustering techniques of sequence features support the notion of extreme conservation of NC-genes across the entire network. Our data show that most genes are under strong purifying selection that is maintained throughout ray-finned fish evolution. Late NC development genes reveal a pattern of increased constraints in more recent lineages. Additionally, seven of the NC-genes showed signs of relaxation of purifying selection in the famously species-rich lineage of cichlid fishes. This suggests that NC genes might have played a role in the adaptive radiation of cichlids by granting flexibility in the development of NC-derived traits-suggesting an important role for NC network architecture during the diversification in vertebrates.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Actinopterygii, teleost, development, ontogeny, gene regulatory network, cichlid, NC-genes, hourglass model

Zitieren

ISO 690

KRATOCHWIL, Claudius F., Laura GEISSLER, Iker IRISARRI, Axel MEYER, 2015. Molecular Evolution of the Neural Crest Regulatory Network in Ray-Finned Fish. In: Genome Biology and Evolution. 2015, 7(11), pp. 3033-3046. eISSN 1759-6653. Available under: doi: 10.1093/gbe/evv200

BibTex

@article{Kratochwil2015Molec-33232,
  year={2015},
  doi={10.1093/gbe/evv200},
  title={Molecular Evolution of the Neural Crest Regulatory Network in Ray-Finned Fish},
  number={11},
  volume={7},
  journal={Genome Biology and Evolution},
  pages={3033--3046},
  author={Kratochwil, Claudius F. and Geißler, Laura and Irisarri, Iker and Meyer, Axel}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/33232">
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-03-04T09:13:50Z</dc:date>
    <dc:creator>Geißler, Laura</dc:creator>
    <dc:contributor>Kratochwil, Claudius F.</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33232/3/Kratochwil_0-312895.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Irisarri, Iker</dc:contributor>
    <dc:creator>Kratochwil, Claudius F.</dc:creator>
    <dc:language>eng</dc:language>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33232"/>
    <dc:contributor>Meyer, Axel</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-03-04T09:13:50Z</dcterms:available>
    <dc:contributor>Geißler, Laura</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33232/3/Kratochwil_0-312895.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:issued>2015</dcterms:issued>
    <dcterms:title>Molecular Evolution of the Neural Crest Regulatory Network in Ray-Finned Fish</dcterms:title>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc/4.0/"/>
    <dc:creator>Meyer, Axel</dc:creator>
    <dc:rights>Attribution-NonCommercial 4.0 International</dc:rights>
    <dc:creator>Irisarri, Iker</dc:creator>
    <dcterms:abstract xml:lang="eng">Gene regulatory networks (GRN) are central to developmental processes. They are composed of transcription factors and signaling molecules orchestrating gene expression modules that tightly regulate the development of organisms. The neural crest (NC) is a multipotent cell population that is considered a key innovation of vertebrates. Its derivatives contribute to shaping the astounding morphological diversity of jaws, teeth, head skeleton, or pigmentation. Here, we study the molecular evolution of the NC GRN by analyzing patterns of molecular divergence for a total of 36 genes in 16 species of bony fishes. Analyses of nonsynonymous to synonymous substitution rate ratios (dN/dS) support patterns of variable selective pressures among genes deployed at different stages of NC development, consistent with the developmental hourglass model. Model-based clustering techniques of sequence features support the notion of extreme conservation of NC-genes across the entire network. Our data show that most genes are under strong purifying selection that is maintained throughout ray-finned fish evolution. Late NC development genes reveal a pattern of increased constraints in more recent lineages. Additionally, seven of the NC-genes showed signs of relaxation of purifying selection in the famously species-rich lineage of cichlid fishes. This suggests that NC genes might have played a role in the adaptive radiation of cichlids by granting flexibility in the development of NC-derived traits-suggesting an important role for NC network architecture during the diversification in vertebrates.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja