rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean

Stecher, Anique; Neuhaus, Stefan; Lange, Benjamin; Frickenhaus, Stephan; Beszteri, Bánk; Kroth, Peter G.; Valentin, Klaus

rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean

Dateien

Stecher_0-313590.pdfGröße: 2.28 MBDownloads: 272

Datum

2016

Autor:innen

Stecher, Anique

Neuhaus, Stefan

Lange, Benjamin

Frickenhaus, Stephan

Beszteri, Bánk

Kroth, Peter G.

Valentin, Klaus

Open Access-Veröffentlichung

Open Access Hybrid

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

European Journal of Phycology. 2016, 51(1), pp. 31-46. ISSN 0967-0262. eISSN 1469-4433. Available under: doi: 10.1080/09670262.2015.1077395

Zusammenfassung

Sea ice is a large and diverse ecosystem contributing significantly to primary production in ice-covered regions. In the Arctic Ocean, sea ice consists of mixed multi-year ice (MYI), often several metres thick, and thinner first-year ice (FYI). Current global warming is most severe in Arctic regions; as a consequence, summer sea ice cover is decreasing and MYI is disappearing at an alarming rate. Despite its apparent hostility, sea ice is inhabited by a diverse microbial community of bacteria and protists, many of which are photosynthetic. Here we present an assessment of eukaryotic biodiversity in MYI and FYI from the central Arctic Ocean using high-throughput 454 sequencing of 18S rRNA and rDNA amplicons. We compared the rDNA-based ‘total’ biodiversity with the ‘active’ biodiversity from rRNA amplicons and found differences between them including an over-representation of Ciliophora, Bicosoecida and Bacillariophyceae operational taxonomic units (OTUs) in the active part of the community. Differences between the two libraries are more pronounced at the lower taxonomic level: certain genera, such as Melosira, are more abundant in the rRNA library, indicating activity of these genera. Furthermore, we found that one FYI station showed a higher activity of potential grazers which was probably due to the advanced stage of melt evident by higher ice temperatures and highly porous ice compared with the other stations.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

STECHER, Anique, Stefan NEUHAUS, Benjamin LANGE, Stephan FRICKENHAUS, Bánk BESZTERI, Peter G. KROTH, Klaus VALENTIN, 2016. rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean. In: European Journal of Phycology. 2016, 51(1), pp. 31-46. ISSN 0967-0262. eISSN 1469-4433. Available under: doi: 10.1080/09670262.2015.1077395

BibTex

@article{Stecher2016based-32868,
  year={2016},
  doi={10.1080/09670262.2015.1077395},
  title={rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean},
  number={1},
  volume={51},
  issn={0967-0262},
  journal={European Journal of Phycology},
  pages={31--46},
  author={Stecher, Anique and Neuhaus, Stefan and Lange, Benjamin and Frickenhaus, Stephan and Beszteri, Bánk and Kroth, Peter G. and Valentin, Klaus}
}

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Universitätsbibliographie

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