Ternary structure reveals mechanism of a membrane diacylglycerol kinase

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2015
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Li, Dianfan
Stansfeld, Phillip J.
Sansom, Mark S. P.
Keogh, Aaron
Vogeley, Lutz
Howe, Nicole
Lyons, Joseph A.
Aragao, David
Fromme, Petra
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Nature Communications. 2015, 6, 10140. eISSN 2041-1723. Available under: doi: 10.1038/ncomms10140
Zusammenfassung

Diacylglycerol kinase catalyses the ATP-dependent conversion of diacylglycerol to phosphatidic acid in the plasma membrane of Escherichia coli. The small size of this integral membrane trimer, which has 121 residues per subunit, means that available protein must be used economically to craft three catalytic and substrate-binding sites centred about the membrane/cytosol interface. How nature has accomplished this extraordinary feat is revealed here in a crystal structure of the kinase captured as a ternary complex with bound lipid substrate and an ATP analogue. Residues, identified as essential for activity by mutagenesis, decorate the active site and are rationalized by the ternary structure. The γ-phosphate of the ATP analogue is positioned for direct transfer to the primary hydroxyl of the lipid whose acyl chain is in the membrane. A catalytic mechanism for this unique enzyme is proposed. The active site architecture shows clear evidence of having arisen by convergent evolution.

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570 Biowissenschaften, Biologie
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Biological sciences, Biochemistry, Biophysics
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ISO 690LI, Dianfan, Phillip J. STANSFELD, Mark S. P. SANSOM, Aaron KEOGH, Lutz VOGELEY, Nicole HOWE, Joseph A. LYONS, David ARAGAO, Petra FROMME, Kay DIEDERICHS, 2015. Ternary structure reveals mechanism of a membrane diacylglycerol kinase. In: Nature Communications. 2015, 6, 10140. eISSN 2041-1723. Available under: doi: 10.1038/ncomms10140
BibTex
@article{Li2015Terna-33403,
  year={2015},
  doi={10.1038/ncomms10140},
  title={Ternary structure reveals mechanism of a membrane diacylglycerol kinase},
  volume={6},
  journal={Nature Communications},
  author={Li, Dianfan and Stansfeld, Phillip J. and Sansom, Mark S. P. and Keogh, Aaron and Vogeley, Lutz and Howe, Nicole and Lyons, Joseph A. and Aragao, David and Fromme, Petra and Diederichs, Kay},
  note={Article Number: 10140}
}
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