Synthesis of DNA oligonucleotides containing 5-(mercaptomethyl)-2′-deoxyuridine moieties

Bornemann, Benjamin; Marx, Andreas

Synthesis of DNA oligonucleotides containing 5-(mercaptomethyl)-2′-deoxyuridine moieties

Dateien

Bornemann_0-329799.pdfGröße: 518.78 KBDownloads: 291

Datum

2006

Autor:innen

Bornemann, Benjamin

Marx, Andreas

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Bioorganic & Medicinal Chemistry. 2006, 14(18), pp. 6235-6238. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2006.05.054

Zusammenfassung

Recently thiolated oligonucleotides have attracted significant interest due to their ability to efficiently undergo stable bond formation with gold nanoparticles and surfaces to form DNA conjugates. In this respect we became interested in the synthesis of oligonucleotides that bear short thioalkyl functions located at the nucleobase. Here we present a strategy for the synthesis of DNA oligonucleotides that bear 5-(mercaptomethyl)-2'-deoxyuridine moieties. The building blocks were synthesized in a straightforward manner from thymidine. Only moderate changes of standard protocols for automated DNA synthesis are required for the generation of modified oligonucleotides containing the thiolated building blocks.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

BORNEMANN, Benjamin, Andreas MARX, 2006. Synthesis of DNA oligonucleotides containing 5-(mercaptomethyl)-2′-deoxyuridine moieties. In: Bioorganic & Medicinal Chemistry. 2006, 14(18), pp. 6235-6238. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2006.05.054

BibTex

@article{Bornemann2006-09-15Synth-33844,
  year={2006},
  doi={10.1016/j.bmc.2006.05.054},
  title={Synthesis of DNA oligonucleotides containing 5-(mercaptomethyl)-2′-deoxyuridine moieties},
  number={18},
  volume={14},
  issn={0968-0896},
  journal={Bioorganic & Medicinal Chemistry},
  pages={6235--6238},
  author={Bornemann, Benjamin and Marx, Andreas}
}

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