The Role of microRNAs in the Repeated Parallel Diversification of Lineages of Midas Cichlid Fish from Nicaragua

Franchini, Paolo; Xiong, Peiwen; Fruciano, Carmelo; Meyer, Axel

The Role of microRNAs in the Repeated Parallel Diversification of Lineages of Midas Cichlid Fish from Nicaragua

Dateien

Franchini_0-347444.pdfGröße: 971.95 KBDownloads: 270

Datum

2016

Angaben zur Forschungsförderung

European Union (EU): 293700

Projekt

Comparative genomics of parallel in repeated adaptive radiations (GenAdap)

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Genome Biology and Evolution. 2016, 8(5), pp. 1543-1555. eISSN 1759-6653. Available under: doi: 10.1093/gbe/evw097

Zusammenfassung

Cichlid fishes are an ideal model system for studying biological diversification because they provide textbook examples of rapid speciation. To date, there has been little focus on the role of gene regulation during cichlid speciation. However, in recent years, gene regulation has been recognized as a powerful force linking diversification in gene function to speciation. Here, we investigated the potential role of miRNA regulation in the diversification of six cichlid species of the Midas cichlid lineage (Amphilophus spp.) inhabiting the Nicaraguan crater lakes. Using several genomic resources, we inferred 236 Midas miRNA genes that were used to predict the miRNA target sites on 8,232 Midas 3'-UTRs. Using population genomic calculations of SNP diversity, we found the miRNA genes to be more conserved than protein coding genes. In contrast to what has been observed in other cichlid fish, but similar to what has been typically found in other groups, we observed genomic signatures of purifying selection on the miRNA targets by comparing these sites with the less conserved nontarget portion of the 3'-UTRs. However, in one species pair that has putatively speciated sympatrically in crater Lake Apoyo, we recovered a different pattern of relaxed purifying selection and high genetic divergence at miRNA targets. Our results suggest that sequence evolution at miRNA binding sites could be a critical genomic mechanism contributing to the rapid phenotypic evolution of Midas cichlids.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

FRANCHINI, Paolo, Peiwen XIONG, Carmelo FRUCIANO, Axel MEYER, 2016. The Role of microRNAs in the Repeated Parallel Diversification of Lineages of Midas Cichlid Fish from Nicaragua. In: Genome Biology and Evolution. 2016, 8(5), pp. 1543-1555. eISSN 1759-6653. Available under: doi: 10.1093/gbe/evw097

BibTex

@article{Franchini2016-06-03micro-35381,
  year={2016},
  doi={10.1093/gbe/evw097},
  title={The Role of microRNAs in the Repeated Parallel Diversification of Lineages of Midas Cichlid Fish from Nicaragua},
  number={5},
  volume={8},
  journal={Genome Biology and Evolution},
  pages={1543--1555},
  author={Franchini, Paolo and Xiong, Peiwen and Fruciano, Carmelo and Meyer, Axel}
}

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Universitätsbibliographie

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