Recruitment of TNF receptor 1 to lipid rafts is essential for TNFalpha-mediated NF-kappaB activation

Legler, Daniel F.; Micheau, Olivier; Doucey, Marie-Agnès; Tschopp, Jürg; Bron, Claude

Recruitment of TNF receptor 1 to lipid rafts is essential for TNFalpha-mediated NF-kappaB activation

Dateien

Legler_0-366195.pdfGröße: 403.85 KBDownloads: 341

Datum

2003

Autor:innen

Legler, Daniel F.

Micheau, Olivier

Doucey, Marie-Agnès

Tschopp, Jürg

Bron, Claude

Open Access-Veröffentlichung

Open Access Green

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Immunity. 2003, 18(5), pp. 655-664. ISSN 1074-7613. eISSN 1097-4180. Available under: doi: 10.1016/S1074-7613(03)00092-X

Zusammenfassung

Engagement of TNF receptor 1 by TNFalpha activates the transcription factor NF-kappaB but can also induce apoptosis. Here we show that upon TNFalpha binding, TNFR1 translocates to cholesterol- and sphingolipid-enriched membrane microdomains, termed lipid rafts, where it associates with the Ser/Thr kinase RIP and the adaptor proteins TRADD and TRAF2, forming a signaling complex. In lipid rafts, TNFR1 and RIP are ubiquitylated. Furthermore, we provide evidence that translocation to lipid rafts precedes ubiquitylation, which leads to the degradation via the proteasome pathway. Interfering with lipid raft organization not only abolishes ubiquitylation but switches TNFalpha signaling from NF-kappaB activation to apoptosis. We suggest that lipid rafts are crucial for the outcome of TNFalpha-activated signaling pathways.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

LEGLER, Daniel F., Olivier MICHEAU, Marie-Agnès DOUCEY, Jürg TSCHOPP, Claude BRON, 2003. Recruitment of TNF receptor 1 to lipid rafts is essential for TNFalpha-mediated NF-kappaB activation. In: Immunity. 2003, 18(5), pp. 655-664. ISSN 1074-7613. eISSN 1097-4180. Available under: doi: 10.1016/S1074-7613(03)00092-X

BibTex

@article{Legler2003Recru-36627,
  year={2003},
  doi={10.1016/S1074-7613(03)00092-X},
  title={Recruitment of TNF receptor 1 to lipid rafts is essential for TNFalpha-mediated NF-kappaB activation},
  number={5},
  volume={18},
  issn={1074-7613},
  journal={Immunity},
  pages={655--664},
  author={Legler, Daniel F. and Micheau, Olivier and Doucey, Marie-Agnès and Tschopp, Jürg and Bron, Claude}
}

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