@article{Valentini2016Analy-37537,
  year={2016},
  doi={10.18632/aging.101022},
  title={Analysis of the machinery and intermediates of the 5hmC-mediated DNA demethylation pathway in aging on samples from the MARK-AGE Study},
  number={9},
  volume={8},
  journal={Aging},
  pages={1896--1922},
  author={Valentini, Elisabetta and Zampieri, Michele and Malavolta, Marco and Bacalini, Maria Giulia and Calabrese, Roberta and Guastafierro, Tiziana and Moreno-Villanueva, Maria and Sindlinger, Thilo and Bürkle, Alexander and Caiafa, Paola}
}

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    <dc:contributor>Bacalini, Maria Giulia</dc:contributor>
    <dc:contributor>Moreno-Villanueva, Maria</dc:contributor>
    <dc:creator>Zampieri, Michele</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Moreno-Villanueva, Maria</dc:creator>
    <dc:contributor>Caiafa, Paola</dc:contributor>
    <dc:contributor>Calabrese, Roberta</dc:contributor>
    <dc:contributor>Bürkle, Alexander</dc:contributor>
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    <dc:contributor>Valentini, Elisabetta</dc:contributor>
    <dc:contributor>Malavolta, Marco</dc:contributor>
    <dc:contributor>Sindlinger, Thilo</dc:contributor>
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    <dc:creator>Bürkle, Alexander</dc:creator>
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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-02-17T07:35:03Z</dc:date>
    <dcterms:abstract xml:lang="eng">Gradual changes in the DNA methylation landscape occur throughout aging virtually in all human tissues. A widespread reduction of 5-methylcytosine (5mC), associated with highly reproducible site-specific hypermethylation, characterizes the genome in aging. Therefore, an equilibrium seems to exist between general and directional deregulating events concerning DNA methylation controllers, which may underpin the age-related epigenetic changes. In this context, 5mC-hydroxylases (TET enzymes) are new potential players. In fact, TETs catalyze the stepwise oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), driving the DNA demethylation process based on thymine DNA glycosylase (TDG)-mediated DNA repair pathway. The present paper reports the expression of DNA hydroxymethylation components, the levels of 5hmC and of its derivatives in peripheral blood mononuclear cells of age-stratified donors recruited in several European countries in the context of the EU Project 'MARK-AGE'. The results provide evidence for an age-related decline of TET1, TET3 and TDG gene expression along with a decrease of 5hmC and an accumulation of 5caC. These associations were independent of confounding variables, including recruitment center, gender and leukocyte composition. The observed impairment of 5hmC-mediated DNA demethylation pathway in blood cells may lead to aberrant transcriptional programs in the elderly.</dcterms:abstract>
    <dc:creator>Guastafierro, Tiziana</dc:creator>
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    <dc:creator>Malavolta, Marco</dc:creator>
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    <dcterms:issued>2016</dcterms:issued>
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    <dc:creator>Bacalini, Maria Giulia</dc:creator>
    <dc:creator>Calabrese, Roberta</dc:creator>
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    <dc:contributor>Guastafierro, Tiziana</dc:contributor>
    <dc:creator>Valentini, Elisabetta</dc:creator>
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    <dc:contributor>Zampieri, Michele</dc:contributor>
    <dcterms:title>Analysis of the machinery and intermediates of the 5hmC-mediated DNA demethylation pathway in aging on samples from the MARK-AGE Study</dcterms:title>
    <dc:creator>Sindlinger, Thilo</dc:creator>
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