Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides

Hottin, Audrey; Betz, Karin; Diederichs, Kay; Marx, Andreas

Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides

Dateien

Hottin_0-398415.pdfGröße: 414.7 KBDownloads: 449

Datum

2017

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Chemistry - A European Journal. 2017, 23(9), pp. 2109-2118. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201604515

Zusammenfassung

Efficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and deoxyuridine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides that prove to be superior substrates for KlenTaq DNA polymerase compared with their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

HOTTIN, Audrey, Karin BETZ, Kay DIEDERICHS, Andreas MARX, 2017. Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides. In: Chemistry - A European Journal. 2017, 23(9), pp. 2109-2118. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201604515

BibTex

@article{Hottin2017-02-10Struc-38270,
  year={2017},
  doi={10.1002/chem.201604515},
  title={Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides},
  number={9},
  volume={23},
  issn={0947-6539},
  journal={Chemistry - A European Journal},
  pages={2109--2118},
  author={Hottin, Audrey and Betz, Karin and Diederichs, Kay and Marx, Andreas}
}

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