Endoplasmic reticulum KDEL-tailed cysteine endopeptidase 1 of Arabidopsis (AtCEP1) is involved in pathogen defense

Höwing, Timo; Huesmann, Christina; Hoefle, Caroline; Nagel, Marie-Kristin; Isono, Erika; Hückelhoven, Ralph; Gietl, Christine

Endoplasmic reticulum KDEL-tailed cysteine endopeptidase 1 of Arabidopsis (AtCEP1) is involved in pathogen defense

Dateien

Hoewing_0-398832.pdfGröße: 1.62 MBDownloads: 467

Datum

2014

Autor:innen

Höwing, Timo

Huesmann, Christina

Hoefle, Caroline

Nagel, Marie-Kristin

Isono, Erika

Hückelhoven, Ralph

Gietl, Christine

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Frontiers in Plant Science. 2014, 5, 58. eISSN 1664-462X. Available under: doi: 10.3389/fpls.2014.00058

Zusammenfassung

Programmed cell death (PCD) is a genetically determined process in all multicellular organisms. Plant PCD is effected by a unique group of papain-type cysteine endopeptidases (CysEP) with a C-terminal KDEL endoplasmic reticulum (ER) retention signal (KDEL CysEP). KDEL CysEPs can be stored as pro-enzymes in ER-derived endomembrane compartments and are released as mature CysEPs in the final stages of organelle disintegration. KDEL CysEPs accept a wide variety of amino acids at the active site, including the glycosylated hydroxyprolines of the extensins that form the basic scaffold of the cell wall. In Arabidopsis, three KDEL CysEPs (AtCEP1, AtCEP2, and AtCEP3) are expressed. Cell- and tissue-specific activities of these three genes suggest that KDEL CysEPs participate in the abscission of flower organs and in the collapse of tissues in the final stage of PCD as well as in developmental tissue remodeling. We observed that AtCEP1 is expressed in response to biotic stress stimuli in the leaf. atcep1 knockout mutants showed enhanced susceptibility to powdery mildew caused by the biotrophic ascomycete Erysiphe cruciferarum. A translational fusion protein of AtCEP1 with a three-fold hemaglutinin-tag and the green fluorescent protein under control of the endogenous AtCEP1 promoter (P_CEP1::pre-pro-3xHA-EGFP-AtCEP1-KDEL) rescued the pathogenesis phenotype demonstrating the function of AtCEP1 in restriction of powdery mildew. The spatiotemporal AtCEP1-reporter expression during fungal infection together with microscopic inspection of the interaction phenotype suggested a function of AtCEP1 in controlling late stages of compatible interaction including late epidermal cell death. Additionally, expression of stress response genes appeared to be deregulated in the interaction of atcep1 mutants and E. cruciferarum. Possible functions of AtCEP1 in restricting parasitic success of the obligate biotrophic powdery mildew fungus are discussed.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

programmed celldeath ; plantimmunity ; sporulation ; haustorium ; cellwall

Zitieren

ISO 690

HÖWING, Timo, Christina HUESMANN, Caroline HOEFLE, Marie-Kristin NAGEL, Erika ISONO, Ralph HÜCKELHOVEN, Christine GIETL, 2014. Endoplasmic reticulum KDEL-tailed cysteine endopeptidase 1 of Arabidopsis (AtCEP1) is involved in pathogen defense. In: Frontiers in Plant Science. 2014, 5, 58. eISSN 1664-462X. Available under: doi: 10.3389/fpls.2014.00058

BibTex

@article{Howing2014Endop-38159,
  year={2014},
  doi={10.3389/fpls.2014.00058},
  title={Endoplasmic reticulum KDEL-tailed cysteine endopeptidase 1 of Arabidopsis (AtCEP1) is involved in pathogen defense},
  volume={5},
  journal={Frontiers in Plant Science},
  author={Höwing, Timo and Huesmann, Christina and Hoefle, Caroline and Nagel, Marie-Kristin and Isono, Erika and Hückelhoven, Ralph and Gietl, Christine},
  note={Article Number: 58}
}

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Universitätsbibliographie

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