Reggie-1 and reggie-2 (flotillins) participate in Rab11a-dependent cargo trafficking, spine synapse formation and LTP-related AMPA receptor (GluA1) surface exposure in mouse hippocampal neurons

Bodrikov, Vsevolod; Pauschert, Aline; Kochlamazashvili, Gaga; Stürmer, Claudia

Reggie-1 and reggie-2 (flotillins) participate in Rab11a-dependent cargo trafficking, spine synapse formation and LTP-related AMPA receptor (GluA1) surface exposure in mouse hippocampal neurons

Dateien

Bodrikov_0-398930.pdfGröße: 3.06 MBDownloads: 705

Datum

2017

Autor:innen

Bodrikov, Vsevolod

Pauschert, Aline

Kochlamazashvili, Gaga

Stürmer, Claudia

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Experimental Neurology. 2017, 289, pp. 31-45. ISSN 0014-4886. eISSN 1090-2430. Available under: doi: 10.1016/j.expneurol.2016.12.007

Zusammenfassung

Reggie-1 and -2 (flotillins) reside at recycling vesicles and promote jointly with Rab11a the targeted delivery of cargo. Recycling is essential for synapse formation suggesting that reggies and Rab11a may regulate the development of spine synapses. Recycling vesicles provide cargo for dendritic growth and recycle surface glutamate receptors (AMPAR, GluA) for long-term potentiation (LTP) induced surface exposure. Here, we show reduced number of spine synapses and impairment of an in vitro correlate of LTP in hippocampal neurons from reggie-1 k.o. (Flot2-/-) mice maturating in culture. These defects apparently result from reduced trafficking of PSD-95 revealed by live imaging of 10 div reggie-1 k.o. (Flot2-/-) neurons and likely impairs co-transport of cargo destined for spines: N-cadherin and the glutamate receptors GluA1 and GluN1. Impaired cargo trafficking and fewer synapses also emerged in reggie-1 siRNA, reggie-2 siRNA, and reggie-1 and -2 siRNA-treated neurons and was in siRNA and k.o. neurons rescued by reggie-1-EGFP and CA-Rab11a-EGFP. While correlative expressional changes of specific synapse proteins were observed in reggie-1 k.o. (Flot2-/-) brains in vivo, this did not occur in neurons maturating in vitro. Our work suggests that reggie-1 and reggie-2 function at Rab11a recycling containers in the transport of PSD-95, N-cadherin, GluA1 and GluN1, and promote (together with significant signaling molecules) spine-directed trafficking, spine synapse formation and the in vitro correlate of LTP.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Correlative expressional changes, In vitro correlate of LTP, Knock out mice, Lipid raft, Recycling, Reggie (flotillin), Spine synapses, Synaptic plasticity

Zitieren

ISO 690

BODRIKOV, Vsevolod, Aline PAUSCHERT, Gaga KOCHLAMAZASHVILI, Claudia STÜRMER, 2017. Reggie-1 and reggie-2 (flotillins) participate in Rab11a-dependent cargo trafficking, spine synapse formation and LTP-related AMPA receptor (GluA1) surface exposure in mouse hippocampal neurons. In: Experimental Neurology. 2017, 289, pp. 31-45. ISSN 0014-4886. eISSN 1090-2430. Available under: doi: 10.1016/j.expneurol.2016.12.007

BibTex

@article{Bodrikov2017Reggi-38200,
  year={2017},
  doi={10.1016/j.expneurol.2016.12.007},
  title={Reggie-1 and reggie-2 (flotillins) participate in Rab11a-dependent cargo trafficking, spine synapse formation and LTP-related AMPA receptor (GluA1) surface exposure in mouse hippocampal neurons},
  volume={289},
  issn={0014-4886},
  journal={Experimental Neurology},
  pages={31--45},
  author={Bodrikov, Vsevolod and Pauschert, Aline and Kochlamazashvili, Gaga and Stürmer, Claudia},
  note={Corrigendum s. https://dx.doi.org/10.1016/j.expneurol.2017.02.016}
}

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Kommentar zur Publikation

Corrigendum s. https://dx.doi.org/10.1016/j.expneurol.2017.02.016
Corrigendum s. https://dx.doi.org/10.1016/j.expneurol.2017.02.016

Universitätsbibliographie

Ja