TrpB2 Enzymes are O-Phospho-l-serine Dependent Tryptophan Synthases

Busch, Florian; Rajendran, Chitra; Mayans, Olga; Löffler, Patrick; Merkl, Rainer; Sterner, Reinhard

TrpB2 Enzymes are O-Phospho-l-serine Dependent Tryptophan Synthases

Dateien

Busch_0-406373.pdfGröße: 186.61 KBDownloads: 365

Datum

2014

Autor:innen

Busch, Florian

Rajendran, Chitra

Mayans, Olga

Löffler, Patrick

Merkl, Rainer

Sterner, Reinhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Biochemistry. 2014, 53(38), pp. 6078-6083. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi500977y

Zusammenfassung

The rapid increase of the number of sequenced genomes asks for the functional annotation of the encoded enzymes. We used a combined computational-structural approach to determine the function of the TrpB2 subgroup of the tryptophan synthase β chain/β chain-like TrpB1-TrpB2 family (IPR023026). The results showed that TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the l-serine dependent synthesis of tryptophan. We found a single residue being responsible for the different substrate specificities of TrpB1 and TrpB2 and confirmed this finding by mutagenesis studies and crystallographic analysis of a TrpB2 enzyme with bound O-phospho-l-serine.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

BUSCH, Florian, Chitra RAJENDRAN, Olga MAYANS, Patrick LÖFFLER, Rainer MERKL, Reinhard STERNER, 2014. TrpB2 Enzymes are O-Phospho-l-serine Dependent Tryptophan Synthases. In: Biochemistry. 2014, 53(38), pp. 6078-6083. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi500977y

BibTex

@article{Busch2014-09-30TrpB2-38702,
  year={2014},
  doi={10.1021/bi500977y},
  title={TrpB2 Enzymes are O-Phospho-l-serine Dependent Tryptophan Synthases},
  number={38},
  volume={53},
  issn={0006-2960},
  journal={Biochemistry},
  pages={6078--6083},
  author={Busch, Florian and Rajendran, Chitra and Mayans, Olga and Löffler, Patrick and Merkl, Rainer and Sterner, Reinhard}
}

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