Technologies of directed protein evolution in vivo

Blagodatski, Artem; Katanaev, Vladimir L.

Technologies of directed protein evolution in vivo

Dateien

katanaev.pdfGröße: 639.39 KBDownloads: 406

Datum

2010

Autor:innen

Blagodatski, Artem

Katanaev, Vladimir L.

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-135735

Link zur Lizenz

Urheberrechtlich geschützt

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Cellular and Molecular Life Sciences. 2010, 68(7), pp. 1207-1214. ISSN 1420-682X. eISSN 1420-9071

Zusammenfassung

Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare prokaryotic and eukaryotic-based systems of directed protein evolution in vivo, highlighting their benefits and current limitations and focusing on the biotechnological potential of vertebrate immune cells for the generation of protein diversity by means of the immunoglobulin diversification machinery.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Directed evolution, Protein engineering, Mutator strain, Cell culture-based systems, Somatic hypermutation, Gene conversion

Zitieren

ISO 690

BLAGODATSKI, Artem, Vladimir L. KATANAEV, 2010. Technologies of directed protein evolution in vivo. In: Cellular and Molecular Life Sciences. 2010, 68(7), pp. 1207-1214. ISSN 1420-682X. eISSN 1420-9071

BibTex

@article{Blagodatski2010Techn-13573,
  year={2010},
  title={Technologies of directed protein evolution in vivo},
  number={7},
  volume={68},
  issn={1420-682X},
  journal={Cellular and Molecular Life Sciences},
  pages={1207--1214},
  author={Blagodatski, Artem and Katanaev, Vladimir L.}
}

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Universitätsbibliographie

Ja