Site-specific DNA labelling by Staudinger ligation

Weisbrod, Samuel H.; Baccaro, Anna; Marx, Andreas

Site-specific DNA labelling by Staudinger ligation

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2011

Autor:innen

Weisbrod, Samuel H.

Baccaro, Anna

Marx, Andreas

Herausgeber:innen

Walker, John

Publikationstyp

Beitrag zu einem Sammelband

Publikationsstatus

Published

Erschienen in

MARK, Sonny S., ed.. Bioconjugation Protocols. Totowa, NJ: Humana Press, 2011, pp. 195-207. Methods in Molecular Biology. 751. ISBN 978-1-61779-150-5. Available under: doi: 10.1007/978-1-61779-151-2_12

Zusammenfassung

Site-specific and chemoselective labeling of DNA is still a difficult task. The Staudinger ligation is a bioorthogonal reaction between azides and phosphines that requires no catalyst to proceed, allowing for mild reaction conditions. The reaction may be extended for site-specific labeling of DNA using azidomodified triphosphates, which can be incorporated site-specifically into DNA strands by DNA polymerases in a template-dependent manner. The azido-modified DNA, in turn, can be labeled by suitable phosphines. This protocol describes (1) the synthesis of an azido-TTP analogue; (2) the enzymatic synthesis of azido-modified DNA; (3) the synthesis of suitable phosphine labels; and (4) the labeling of azido-DNA with biotin–phosphine by Staudinger ligation with approximately 70% conversion.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

Site-specific DNA labeling, DNA modification, Staudinger ligation, Phosphine, Modified triphosphate, Azido thymidine, DNA polymerase, Azido-DNA, Biotin

Zitieren

ISO 690

WEISBROD, Samuel H., Anna BACCARO, Andreas MARX, WALKER, John, ed., 2011. Site-specific DNA labelling by Staudinger ligation. In: MARK, Sonny S., ed.. Bioconjugation Protocols. Totowa, NJ: Humana Press, 2011, pp. 195-207. Methods in Molecular Biology. 751. ISBN 978-1-61779-150-5. Available under: doi: 10.1007/978-1-61779-151-2_12

BibTex

@incollection{Weisbrod2011Sites-14013,
  year={2011},
  doi={10.1007/978-1-61779-151-2_12},
  title={Site-specific DNA labelling by Staudinger ligation},
  number={751},
  isbn={978-1-61779-150-5},
  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in Molecular Biology},
  booktitle={Bioconjugation Protocols},
  pages={195--207},
  editor={Mark, Sonny S.},
  author={Weisbrod, Samuel H. and Baccaro, Anna and Marx, Andreas}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/14013">
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Marx, Andreas</dc:creator>
    <dcterms:title>Site-specific DNA labelling by Staudinger ligation</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-07-19T08:19:27Z</dc:date>
    <dc:contributor>Walker, John</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:creator>Baccaro, Anna</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-07-19T08:19:27Z</dcterms:available>
    <dc:contributor>Baccaro, Anna</dc:contributor>
    <dc:creator>Weisbrod, Samuel H.</dc:creator>
    <dcterms:issued>2011</dcterms:issued>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14013"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Weisbrod, Samuel H.</dc:contributor>
    <dcterms:abstract xml:lang="eng">Site-specific and chemoselective labeling of DNA is still a difficult task. The Staudinger ligation is a bioorthogonal reaction between azides and phosphines that requires no catalyst to proceed, allowing for mild reaction conditions. The reaction may be extended for site-specific labeling of DNA using azidomodified triphosphates, which can be incorporated site-specifically into DNA strands by DNA polymerases in a template-dependent manner. The azido-modified DNA, in turn, can be labeled by suitable phosphines. This protocol describes (1) the synthesis of an azido-TTP analogue; (2) the enzymatic synthesis of azido-modified DNA; (3) the synthesis of suitable phosphine labels; and (4) the labeling of azido-DNA with biotin–phosphine by Staudinger ligation with approximately 70% conversion.</dcterms:abstract>
    <dcterms:bibliographicCitation>Publ. in: Bioconjugation Protocols / ed. by Sonny S. Mark (Methods in Molecular Biology, Vol. 751). Totowa, NJ : Humana Press, 2011, 2. Ed., pp. 195-207</dcterms:bibliographicCitation>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja