RAG2-/- gamma(c)-/- mice transplanted with CD34+ cells from human cord blood show low levels of intestinal engraftment and are resistant to rectal transmission of human immunodeficiency virus
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
Rectal transmission is one of the main routes of infection by human immunodeficiency virus type 1 (HIV-1). To efficiently study transmission mechanisms and prevention strategies, a small animal model permissive for rectal transmission of HIV is mandatory. We tested the susceptibility of RAG2-/- gamma(c)-/- mice transplanted with human cord blood hematopoietic stem cells to rectal infection with HIV. We rectally exposed these humanized mice to cell-free and cell-associated HIV. All mice remained HIV negative as assessed by plasma viral load. The same mice infected intraperitoneally showed high levels of HIV replication. In the gut-associated lymphatic tissue, we found disproportionately smaller numbers of human cells than in other lymphoid organs. This finding may explain the observed resistance to rectal transmission of HIV. To increase the numbers of local HIV target cells and the likelihood of HIV transmission, we treated mice with different proinflammatory stimuli: local application of interleukin-1 , addition of seminal plasma to the inoculum, or induction of colitis with dextran sodium sulfate. These procedures attracted some human leukocytes, but the transmission rate was still very low. The humanized mice showed low levels of human engraftment in the intestinal tract and seem to be resistant to rectal transmission of HIV, and thus they are an unsuitable model for this application.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
HOFER, Ursula, Stefan BAENZIGER, Mathias HEIKENWALDER, Erika SCHLAEPFER, Nadine GEHRE, Stephan REGENASS, Thomas BRUNNER, Roberto SPECK, 2008. RAG2-/- gamma(c)-/- mice transplanted with CD34+ cells from human cord blood show low levels of intestinal engraftment and are resistant to rectal transmission of human immunodeficiency virus. In: Journal of Virology. 2008, 82(24), pp. 12145-12153. ISSN 0022-538X. eISSN 1098-5514. Available under: doi: 10.1128/JVI.01105-08BibTex
@article{Hofer2008-12gamma-14264, year={2008}, doi={10.1128/JVI.01105-08}, title={RAG2-/- gamma(c)-/- mice transplanted with CD34+ cells from human cord blood show low levels of intestinal engraftment and are resistant to rectal transmission of human immunodeficiency virus}, number={24}, volume={82}, issn={0022-538X}, journal={Journal of Virology}, pages={12145--12153}, author={Hofer, Ursula and Baenziger, Stefan and Heikenwalder, Mathias and Schlaepfer, Erika and Gehre, Nadine and Regenass, Stephan and Brunner, Thomas and Speck, Roberto} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/14264"> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:contributor>Regenass, Stephan</dc:contributor> <dcterms:abstract xml:lang="eng">Rectal transmission is one of the main routes of infection by human immunodeficiency virus type 1 (HIV-1). To efficiently study transmission mechanisms and prevention strategies, a small animal model permissive for rectal transmission of HIV is mandatory. We tested the susceptibility of RAG2-/- gamma(c)-/- mice transplanted with human cord blood hematopoietic stem cells to rectal infection with HIV. We rectally exposed these humanized mice to cell-free and cell-associated HIV. All mice remained HIV negative as assessed by plasma viral load. The same mice infected intraperitoneally showed high levels of HIV replication. In the gut-associated lymphatic tissue, we found disproportionately smaller numbers of human cells than in other lymphoid organs. This finding may explain the observed resistance to rectal transmission of HIV. To increase the numbers of local HIV target cells and the likelihood of HIV transmission, we treated mice with different proinflammatory stimuli: local application of interleukin-1 , addition of seminal plasma to the inoculum, or induction of colitis with dextran sodium sulfate. These procedures attracted some human leukocytes, but the transmission rate was still very low. The humanized mice showed low levels of human engraftment in the intestinal tract and seem to be resistant to rectal transmission of HIV, and thus they are an unsuitable model for this application.</dcterms:abstract> <dcterms:issued>2008-12</dcterms:issued> <dc:rights>terms-of-use</dc:rights> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14264/2/2008_Hofer_%20J.%20Virol_08.pdf"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14264/2/2008_Hofer_%20J.%20Virol_08.pdf"/> <dc:creator>Gehre, Nadine</dc:creator> <dc:creator>Heikenwalder, Mathias</dc:creator> <dc:creator>Hofer, Ursula</dc:creator> <dc:creator>Schlaepfer, Erika</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14264"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-19T09:26:31Z</dc:date> <dc:contributor>Speck, Roberto</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Baenziger, Stefan</dc:contributor> <dc:contributor>Gehre, Nadine</dc:contributor> <dc:creator>Speck, Roberto</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:creator>Baenziger, Stefan</dc:creator> <dc:creator>Regenass, Stephan</dc:creator> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:contributor>Heikenwalder, Mathias</dc:contributor> <dc:contributor>Schlaepfer, Erika</dc:contributor> <dc:contributor>Hofer, Ursula</dc:contributor> <dc:creator>Brunner, Thomas</dc:creator> <dcterms:bibliographicCitation>First publ. in: Journal of Virology ; 82 (2008), 24. - pp. 12145-12153</dcterms:bibliographicCitation> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:title>RAG2-/- gamma(c)-/- mice transplanted with CD34+ cells from human cord blood show low levels of intestinal engraftment and are resistant to rectal transmission of human immunodeficiency virus</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-19T09:26:31Z</dcterms:available> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> </rdf:Description> </rdf:RDF>