Active caspase 3 and DNA fragmentation as markers for apoptotic cell death in primary and metastatic liver tumours

Karamitopoulou, Eva; Cioccari, Luca; Jakob, Sabine; Vallan, Claudio; Schaffner, Thomas; Zimmermann, Arthur; Brunner, Thomas

Active caspase 3 and DNA fragmentation as markers for apoptotic cell death in primary and metastatic liver tumours

Dateien

Karamitopoulou_142713.pdfGröße: 226.21 KBDownloads: 362

Datum

2007

Autor:innen

Karamitopoulou, Eva

Cioccari, Luca

Jakob, Sabine

Vallan, Claudio

Schaffner, Thomas

Zimmermann, Arthur

Brunner, Thomas

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Pathology. 2007, 39(6), pp. 558-564. ISSN 1465-3931. Available under: doi: 10.1080/00313020701684375

Zusammenfassung

Aims: The induction of tumour cell death by apoptosis is a
major goal of cancer therapy and the in situ detection of
apoptosis in tumour tissue has become an important
diagnostic parameter. Different apoptosis detection methods
assess distinct biochemical processes in the dying cell. Thus,
their direct comparison is mandatory to evaluate their
diagnostic value. The aim of this study was to compare the
immunohistochemical detection of active caspase 3 and
single-stranded DNA in primary and metastatic liver tumours
as markers of apoptotic cell death.
Methods: We studied detection of active caspase 3 and
single-stranded DNA in 20 primary hepatocellular carcinomas
(HCC) and 20 liver metastases from colorectal
carcinomas (CRC) using immunohistochemistry on paraffin
sections.
Results: Our results reveal that both methods are suitable
and sensitive techniques for the in situ detection of
apoptosis, however, they also demonstrate that immunohistochemistry
for active caspase 3 and single-stranded DNA
have differential sensitivities in HCC and CRC.
Conclusion: The sensitivity of apoptosis detection using
immunohistochemistry for active caspase 3 and singlestranded
DNA may be tumour cell type dependent.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Apoptosis, Caspases, DNA fragmentation, Hepatocellular carcinoma, Colorectal carcinoma, Immunohistochemistry

Zitieren

ISO 690

KARAMITOPOULOU, Eva, Luca CIOCCARI, Sabine JAKOB, Claudio VALLAN, Thomas SCHAFFNER, Arthur ZIMMERMANN, Thomas BRUNNER, 2007. Active caspase 3 and DNA fragmentation as markers for apoptotic cell death in primary and metastatic liver tumours. In: Pathology. 2007, 39(6), pp. 558-564. ISSN 1465-3931. Available under: doi: 10.1080/00313020701684375

BibTex

@article{Karamitopoulou2007-12Activ-14271,
  year={2007},
  doi={10.1080/00313020701684375},
  title={Active caspase 3 and DNA fragmentation as markers for apoptotic cell death in primary and metastatic liver tumours},
  number={6},
  volume={39},
  issn={1465-3931},
  journal={Pathology},
  pages={558--564},
  author={Karamitopoulou, Eva and Cioccari, Luca and Jakob, Sabine and Vallan, Claudio and Schaffner, Thomas and Zimmermann, Arthur and Brunner, Thomas}
}

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Universitätsbibliographie

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