RNA quadruplex-based modulation of gene expression

Wieland, Markus; Hartig, Jörg S.

RNA quadruplex-based modulation of gene expression

Dateien

wieland_156240.pdfGröße: 622.57 KBDownloads: 211

Datum

2007

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Chemistry & Biology. 2007, 14(7), pp. 757-763. ISSN 1074-5521. Available under: doi: 10.1016/j.chembiol.2007.06.005

Zusammenfassung

RNA-based modules such as riboswitches represent straightforward and simplified approaches for the regulation of gene expression, as no additional proteins are needed. G-rich sequences are known to adopt stable four-stranded structures, and such quadruplexes have been suspected to play important roles in key functions such as the control of gene expression. Here we demonstrate that RNA quadruplexes readily form in vivo. We have constructed mRNA-based G-rich elements that mask the ribosome binding site by folding into four-stranded structures. The suppression of gene expression correlates with the stability of inserted G quadruplexes. Moreover, quadruplexes with moderate stability respond to changes in temperature, thus behaving as artificial RNA thermometers. In conclusion, we introduce tuneable mRNA-based devices that enable modulation of gene expression by a predictable but thus far unknown mechanism.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

Chembio, RNA

Zitieren

ISO 690

WIELAND, Markus, Jörg S. HARTIG, 2007. RNA quadruplex-based modulation of gene expression. In: Chemistry & Biology. 2007, 14(7), pp. 757-763. ISSN 1074-5521. Available under: doi: 10.1016/j.chembiol.2007.06.005

BibTex

@article{Wieland2007-07quadr-15624,
  year={2007},
  doi={10.1016/j.chembiol.2007.06.005},
  title={RNA quadruplex-based modulation of gene expression},
  number={7},
  volume={14},
  issn={1074-5521},
  journal={Chemistry & Biology},
  pages={757--763},
  author={Wieland, Markus and Hartig, Jörg S.}
}

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