Long-Range Distance Determination in a DNA Model System inside Xenopus Laevis Oocytes by In-Cell Spin-Label EPR

Azarkh, Mykhailo; Okle, Oliver; Holder, Isabelle T.; Singh, Vijay; Hartig, Jörg S.; Dietrich, Daniel R.; Drescher, Malte

Long-Range Distance Determination in a DNA Model System inside Xenopus Laevis Oocytes by In-Cell Spin-Label EPR

Dateien

Drescher etal.pdfGröße: 1.82 MBDownloads: 1033

Datum

2011

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

ChemBioChem. 2011, 12(13), pp. 1992-1995. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201100281

Zusammenfassung

DEER strides: The development of analytical tools for elucidating native structures inside cells is an ongoing challenge. Conformational changes of DNA upon injection into living cells (see figure) can be detected by a combination of site-directed spin labeling and DEER (double electron–electron resonance) spectroscopy. This technique offers structural information on the nm scale, in cellulo, background-free and at low concentrations.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

EPR distance measurements, EPR spectroscopy, in-cell DEER, spin labeling, structure elucidation

Zitieren

ISO 690

AZARKH, Mykhailo, Oliver OKLE, Isabelle T. HOLDER, Vijay SINGH, Jörg S. HARTIG, Daniel R. DIETRICH, Malte DRESCHER, 2011. Long-Range Distance Determination in a DNA Model System inside Xenopus Laevis Oocytes by In-Cell Spin-Label EPR. In: ChemBioChem. 2011, 12(13), pp. 1992-1995. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201100281

BibTex

@article{Azarkh2011-09-05LongR-15673,
  year={2011},
  doi={10.1002/cbic.201100281},
  title={Long-Range Distance Determination in a DNA Model System inside Xenopus Laevis Oocytes by In-Cell Spin-Label EPR},
  number={13},
  volume={12},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={1992--1995},
  author={Azarkh, Mykhailo and Okle, Oliver and Holder, Isabelle T. and Singh, Vijay and Hartig, Jörg S. and Dietrich, Daniel R. and Drescher, Malte}
}

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