Screening of molecular interactions using reporter hammerhead ribozymes

Hartig, Jörg S.; Famulok, Michael

Screening of molecular interactions using reporter hammerhead ribozymes

Dateien

Hartig_156817.pdfGröße: 818.16 KBDownloads: ?

Datum

2008

Autor:innen

Hartig, Jörg S.

Famulok, Michael

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie
Zukunftskolleg

Publikationstyp

Beitrag zu einem Sammelband

Publikationsstatus

Published

Erschienen in

MARX, Andreas, ed., Oliver SEITZ, ed.. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols. Totowa, NJ: Humana Press, 2008, pp. 251-263. Methods in Molecular Biology. 429. ISBN 978-1-58829-700-6. Available under: doi: 10.1007/978-1-60327-040-3_18

Zusammenfassung

The characterization of molecular interactions is a central task in modern life sciences. Applications such as drug screening in pharmaceutics or the elucidation of biomolecular interactions in molecular biology rely on efficient methods to search for interacting partners. Here, we describe a novel technique that utilizes hammerhead ribozymes to signal molecular interactions. The ribozyme is modified by a domain that specifically binds to a target molecule such as a protein. Upon binding of the target, the catalytic activity of the ribozyme is changed, allowing for detection of the presence as well as the occurrence of interactions of the targeted ligand. The assay can be performed in high-throughput format by employing double-labeled ribozyme substrates, hence being well suited for drug-screening applications. The detection proceeds rapidly and in real-time. Moreover, the technique neither requires labeling of the target molecule nor the potential interaction partners or analytes since an indirect readout is facilitated by switching the catalytic activity of a reporter ribozyme. The assay can be utilized to sense a broad variety of biomolecular interactions, and is very sensitive due to signal amplification by the ribozyme reaction

Fachgebiet (DDC)

540 Chemie

Schlagwörter

Hammerhead ribozyme, aptamer, RNA reporter, drug-screening, protein-interactions, FRET-assay, signal amplification

Zitieren

ISO 690

HARTIG, Jörg S., Michael FAMULOK, 2008. Screening of molecular interactions using reporter hammerhead ribozymes. In: MARX, Andreas, ed., Oliver SEITZ, ed.. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols. Totowa, NJ: Humana Press, 2008, pp. 251-263. Methods in Molecular Biology. 429. ISBN 978-1-58829-700-6. Available under: doi: 10.1007/978-1-60327-040-3_18

BibTex

@incollection{Hartig2008Scree-15681,
  year={2008},
  doi={10.1007/978-1-60327-040-3_18},
  title={Screening of molecular interactions using reporter hammerhead ribozymes},
  number={429},
  isbn={978-1-58829-700-6},
  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in Molecular Biology},
  booktitle={Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols},
  pages={251--263},
  editor={Marx, Andreas and Seitz, Oliver},
  author={Hartig, Jörg S. and Famulok, Michael}
}

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Universitätsbibliographie

Ja