Artificial Ribozyme-Based Regulators of Gene Expression

Holder, Isabelle T.; Hartig, Jörg S.

Artificial Ribozyme-Based Regulators of Gene Expression

Dateien

seemann_157776.pdfGröße: 794 KBDownloads: 462

Datum

2011

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Synlett. 2011, 2011(11), pp. 1486-1494. ISSN 0936-5214. eISSN 1437-2096. Available under: doi: 10.1055/s-0030-1260583

Zusammenfassung

The development of RNA-based switches of gene expression is summarized. Most switches are based on the Schistosoma mansonii hammerhead ribozyme (HHR), a self-cleaving RNA sequence that is inserted into an mRNA. Control of HHR self-cleavage is achieved by the attachment of an aptamer domain to the HHR scaffold. External addition of a small-molecule ligand regulates catalytic activity of the ribozyme and hence gene expression. These so-called aptazymes are suited to control several classes of RNA. In addition to mRNA, we have incorporated artificial RNA switches into tRNA as well as the bacterial 16S rRNA. In addition, the presented switches should be widely applicable as demonstrated by experiments in bacteria, yeast, and mammals.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

HOLDER, Isabelle T., Jörg S. HARTIG, 2011. Artificial Ribozyme-Based Regulators of Gene Expression. In: Synlett. 2011, 2011(11), pp. 1486-1494. ISSN 0936-5214. eISSN 1437-2096. Available under: doi: 10.1055/s-0030-1260583

BibTex

@article{Holder2011Artif-15777,
  year={2011},
  doi={10.1055/s-0030-1260583},
  title={Artificial Ribozyme-Based Regulators of Gene Expression},
  number={11},
  volume={2011},
  issn={0936-5214},
  journal={Synlett},
  pages={1486--1494},
  author={Holder, Isabelle T. and Hartig, Jörg S.}
}

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