Evolving a thermostable DNA polymerase that amplifies from highly damaged templates
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2007
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Angewandte Chemie International Edition. 2007, 46(17), pp. 3115-3117. ISSN 1433-7851. Available under: doi: 10.1002/anie.200603987
Zusammenfassung
The foreign lesion: DNAs with damage generated, for example, by UV light are not good targets for standard replicative DNA polymerases. A DNA polymerase has been derived by directed evolution that faithfully amplifies light-damaged DNA. Interestingly, a single mutation is sufficient for this property
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
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directed evolution, DNA damage, DNA polymerase, polymerase chain reaction
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GLÖCKNER, Christian, Katharina B. SAUTER, Andreas MARX, 2007. Evolving a thermostable DNA polymerase that amplifies from highly damaged templates. In: Angewandte Chemie International Edition. 2007, 46(17), pp. 3115-3117. ISSN 1433-7851. Available under: doi: 10.1002/anie.200603987BibTex
@article{Glockner2007Evolv-16698, year={2007}, doi={10.1002/anie.200603987}, title={Evolving a thermostable DNA polymerase that amplifies from highly damaged templates}, number={17}, volume={46}, issn={1433-7851}, journal={Angewandte Chemie International Edition}, pages={3115--3117}, author={Glöckner, Christian and Sauter, Katharina B. and Marx, Andreas} }
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