Evolving a thermostable DNA polymerase that amplifies from highly damaged templates

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2007
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Glöckner, Christian
Sauter, Katharina B.
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Angewandte Chemie International Edition. 2007, 46(17), pp. 3115-3117. ISSN 1433-7851. Available under: doi: 10.1002/anie.200603987
Zusammenfassung

The foreign lesion: DNAs with damage generated, for example, by UV light are not good targets for standard replicative DNA polymerases. A DNA polymerase has been derived by directed evolution that faithfully amplifies light-damaged DNA. Interestingly, a single mutation is sufficient for this property

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Fachgebiet (DDC)
540 Chemie
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directed evolution, DNA damage, DNA polymerase, polymerase chain reaction
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ISO 690GLÖCKNER, Christian, Katharina B. SAUTER, Andreas MARX, 2007. Evolving a thermostable DNA polymerase that amplifies from highly damaged templates. In: Angewandte Chemie International Edition. 2007, 46(17), pp. 3115-3117. ISSN 1433-7851. Available under: doi: 10.1002/anie.200603987
BibTex
@article{Glockner2007Evolv-16698,
  year={2007},
  doi={10.1002/anie.200603987},
  title={Evolving a thermostable DNA polymerase that amplifies from highly damaged templates},
  number={17},
  volume={46},
  issn={1433-7851},
  journal={Angewandte Chemie International Edition},
  pages={3115--3117},
  author={Glöckner, Christian and Sauter, Katharina B. and Marx, Andreas}
}
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