Distinct Roles for Two Histamine Receptors (hclA and hclB) at the Drosophila Photoreceptor Synapse

Pantazis, Antonios; Segaran, Ashvina; Liu, Che-Hsiung; Nikolaev, Anton; Rister, Jens; Thum, Andreas; Roeder, Thomas; Semenov, Eugene; Juusola, Mikko; Hardie, Roger C.

Distinct Roles for Two Histamine Receptors (hclA and hclB) at the Drosophila Photoreceptor Synapse

Dateien

Thum etal.pdfGröße: 2.55 MBDownloads: 353

Datum

2008

Autor:innen

Pantazis, Antonios

Segaran, Ashvina

Liu, Che-Hsiung

Nikolaev, Anton

Rister, Jens

Thum, Andreas

Roeder, Thomas

Semenov, Eugene

Juusola, Mikko

Hardie, Roger C.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Neuroscience. 2008, 28(29), pp. 7250-7259. ISSN 0270-6474. eISSN 1529-2401. Available under: doi: 10.1523/JNEUROSCI.1654-08.2008

Zusammenfassung

Histamine (HA) is the photoreceptor neurotransmitter in arthropods, directly gating chloride channels on large monopolar cells (LMCs), postsynaptic to photoreceptors in the lamina. Two histamine-gated channel genes that could contribute to this channel in Drosophila are hclA (also known as ort) and hclB (also known as hisCl1), both encoding novel members of the Cys-loop receptor superfamily. Drosophila S2 cells transfected with these genes expressed both homomeric and heteromeric histamine-gated chloride channels. The electrophysiological properties of these channels were compared with those from isolated Drosophila LMCs.HCLAhomomershad nearly identicalHA sensitivity to the native receptors (EC ₅₀ = 25 µM). Single-channel analysis revealed further close similarity in terms of single-channel kinetics and subconductance states (~25, 40, and 60 pS, the latter strongly voltage dependent). In contrast, HCLB homomers and heteromeric receptors were more sensitive to HA (EC₅₀ = 14 and 1.2µM, respectively), with much smaller single-channel conductances (~4 pS). Null mutations of hclA (ort^US6096) abolished the synaptic transients in the electroretinograms (ERGs). Surprisingly, theERG“on” transients in hclB mutants transients were approximately twofold enhanced, whereas intracellular recordings from their LMCs revealed altered responses with slower kinetics. However, HCLB expression within the lamina, assessed by both a GFP (green fluorescent protein) reporter gene strategy and mRNA tagging, was exclusively localized to the glia cells, whereas HCLA expression was confirmed in the LMCs. Our results suggest that the native receptor at the LMC synapse is an HCLA homomer, whereas HCLB signaling via the lamina glia plays a previously unrecognized role in shaping the LMC postsynaptic response.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

ligand-gated ion channel, chloride channel, retina, vision, LMC, lamina, glia, ort

Zitieren

ISO 690

PANTAZIS, Antonios, Ashvina SEGARAN, Che-Hsiung LIU, Anton NIKOLAEV, Jens RISTER, Andreas THUM, Thomas ROEDER, Eugene SEMENOV, Mikko JUUSOLA, Roger C. HARDIE, 2008. Distinct Roles for Two Histamine Receptors (hclA and hclB) at the Drosophila Photoreceptor Synapse. In: Journal of Neuroscience. 2008, 28(29), pp. 7250-7259. ISSN 0270-6474. eISSN 1529-2401. Available under: doi: 10.1523/JNEUROSCI.1654-08.2008

BibTex

@article{Pantazis2008-07-16Disti-17149,
  year={2008},
  doi={10.1523/JNEUROSCI.1654-08.2008},
  title={Distinct Roles for Two Histamine Receptors (hclA and hclB) at the Drosophila Photoreceptor Synapse},
  number={29},
  volume={28},
  issn={0270-6474},
  journal={Journal of Neuroscience},
  pages={7250--7259},
  author={Pantazis, Antonios and Segaran, Ashvina and Liu, Che-Hsiung and Nikolaev, Anton and Rister, Jens and Thum, Andreas and Roeder, Thomas and Semenov, Eugene and Juusola, Mikko and Hardie, Roger C.}
}

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