A Non-Motor Microtubule Binding Site Is Essential for the High Processivity and Mitotic Function of Kinesin-8 Kif18A

Mayr, Monika; Storch, Marko; Howard, Jonathon; Mayer, Thomas U.

A Non-Motor Microtubule Binding Site Is Essential for the High Processivity and Mitotic Function of Kinesin-8 Kif18A

Dateien

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Datum

2011

Autor:innen

Mayr, Monika

Storch, Marko

Howard, Jonathon

Mayer, Thomas U.

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

PLoS ONE. 2011, 6(11), e27471. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0027471

Zusammenfassung

BACKGROUND:

Members of the kinesin-8 subfamily are plus end-directed molecular motors that accumulate at the plus-ends of kinetochore-microtubules (kt-MTs) where they regulate MT dynamics. Loss of vertebrate kinesin-8 function induces hyperstable MTs and elongated mitotic spindles accompanied by severe chromosome congression defects. It has been reported that the motility of human kinesin-8, Kif18A, is required for its accumulation at the plus tips of kt-MTs.

METHODOLOGY/FINDINGS:

Here, we investigate how Kif18A localizes to the plus-ends of kt-MTs. We find that Kif18A lacking its C-terminus does not accumulate on the tips of kt-MTs and fails to fulfill its mitotic function. In vitro studies reveal that Kif18A possesses a non-motor MT binding site located within its C-proximal 121 residues. Using single molecule measurements we find that Kif18A is a highly processive motor and, furthermore, that the C-terminal tail is essential for the high processivity of Kif18A.

CONCLUSION/SIGNIFICANCE:

These results show that Kif18A like its yeast orthologue is a highly processive motor. The ability of Kif18A to walk on MTs for a long distance without dissociating depends on a non-motor MT binding site located at the C-terminus of Kif18A. This C-proximal tail of Kif18A is essential for its plus-end accumulation and mitotic function. These findings advance our understanding of how Kif18A accumulates at the tips of kt-MTs to fulfill its function in mitosis.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Kif18A, KInesin, Microtubule, Mitose, Chromosomenbande Zellteilung, Mikrotubuli, Kinesin

Zitieren

ISO 690

MAYR, Monika, Marko STORCH, Jonathon HOWARD, Thomas U. MAYER, 2011. A Non-Motor Microtubule Binding Site Is Essential for the High Processivity and Mitotic Function of Kinesin-8 Kif18A. In: PLoS ONE. 2011, 6(11), e27471. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0027471

BibTex

@article{Mayr2011NonMo-17239,
  year={2011},
  doi={10.1371/journal.pone.0027471},
  title={A Non-Motor Microtubule Binding Site Is Essential for the High Processivity and Mitotic Function of Kinesin-8 Kif18A},
  number={11},
  volume={6},
  journal={PLoS ONE},
  author={Mayr, Monika and Storch, Marko and Howard, Jonathon and Mayer, Thomas U.},
  note={Article Number: e27471}
}

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