Prion protein promotes growth cone development through reggie/flotillin-dependent N-cadherin trafficking

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Journal of Neuroscience. 2011, 31(49), pp. 18013-18025. ISSN 0270-6474. eISSN 1529-2401. Available under: doi: 10.1523/JNEUROSCI.4729-11.2011
Zusammenfassung

The role of prion protein (PrP) is insufficiently understood partially because PrP-deficient (-/-) neurons from C57BL/6J mice seem to differentiate normally and are functionally mildly impaired. Here, we reassessed this notion and, unexpectedly, discovered that PrP(-/-) hippocampal growth cones were abnormally small and poor in filopodia and cargo-containing vesicles. Based on our findings that PrP-PrP trans-interaction recruits E-cadherin to cell contact sites and reggie microdomains, and that reggies are essential for growth by regulating membrane trafficking, we reasoned that PrP and reggie might promote cargo (N-cadherin) delivery via PrP-reggie-connected signaling upon PrP activation (by PrP-Fc-induced trans-interaction). In wild-type but not PrP(-/-) neurons, PrP activation led to (1) enhanced PrP-reggie cocluster formation, (2) reggie-associated fyn and MAP kinase activation, (3) Exo70 and N-cadherin (cargo) recruitment to reggie, (4) the preference of the growth cone for PrP-Fc as substrate, and (5) longer neurites. Conversely, PrP-reggie-induced N-cadherin recruitment was blocked by mutant TC10, the GTPase downstream of reggie, triggering exocyst-assisted cargo delivery. This implies that PrP functions in reggie-mediated signaling and cargo trafficking, thus promoting growth cone complexity and vitality and thereby growth cone elongation.

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ISO 690BODRIKOV, Vsevolod, Gonzalo SOLIS PADILLA, Claudia STÃœRMER, 2011. Prion protein promotes growth cone development through reggie/flotillin-dependent N-cadherin trafficking. In: Journal of Neuroscience. 2011, 31(49), pp. 18013-18025. ISSN 0270-6474. eISSN 1529-2401. Available under: doi: 10.1523/JNEUROSCI.4729-11.2011
BibTex
@article{Bodrikov2011-12-07Prion-17473,
  year={2011},
  doi={10.1523/JNEUROSCI.4729-11.2011},
  title={Prion protein promotes growth cone development through reggie/flotillin-dependent N-cadherin trafficking},
  number={49},
  volume={31},
  issn={0270-6474},
  journal={Journal of Neuroscience},
  pages={18013--18025},
  author={Bodrikov, Vsevolod and Solis Padilla, Gonzalo and Stürmer, Claudia}
}
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