Biomarker candidates of Chlamydophila pneumoniae proteins and protein fragments identified by affinity-proteomics using FTICR-MS and LC-MS/MS

Susnea, Iuliana; Bunk, Sebastian; Wendel, Albrecht; Hermann, Corinna; Przybylski, Michael

Biomarker candidates of Chlamydophila pneumoniae proteins and protein fragments identified by affinity-proteomics using FTICR-MS and LC-MS/MS

Dateien

Przybylski.pdfGröße: 2.27 MBDownloads: 304

Datum

2011

Autor:innen

Susnea, Iuliana

Bunk, Sebastian

Wendel, Albrecht

Hermann, Corinna

Przybylski, Michael

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie
Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of The American Society for Mass Spectrometry. 2011, 22(4), pp. 784-788. ISSN 1044-0305. eISSN 1879-1123. Available under: doi: 10.1007/s13361-011-0082-3

Zusammenfassung

We report here an affinity-proteomics approach that combines 2D-gel electrophoresis and immunoblotting with high performance mass spectrometry to the identification of both full length protein antigens and antigenic fragments of Chlamydophila pneumoniae (C. pneumoniae). The present affinity-mass spectrometry approach effectively utilized high resolution FTICR mass spectrometry and LC-tandem-MS for protein identification, and enabled the identification of several new highly antigenic C. pneumoniae proteins that were not hitherto reported or previously detected only in other Chlamydia species, such as Chlamydia trachomatis. Moreover, high resolution affinity-MS provided the identification of several neo-antigenic protein fragments containing N- and C-terminal, and central domains such as fragments of the membrane protein Pmp21 and the secreted chlamydial proteasome-like factor (Cpaf), representing specific biomarker candidates.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

Affinity-proteomics, Chlamydia pneumoniae, protein biomarker candidates, high resolution mass spectrometry, LC- tandem mass spectrometry, neoantigenic protein fragments

Zitieren

ISO 690

SUSNEA, Iuliana, Sebastian BUNK, Albrecht WENDEL, Corinna HERMANN, Michael PRZYBYLSKI, 2011. Biomarker candidates of Chlamydophila pneumoniae proteins and protein fragments identified by affinity-proteomics using FTICR-MS and LC-MS/MS. In: Journal of The American Society for Mass Spectrometry. 2011, 22(4), pp. 784-788. ISSN 1044-0305. eISSN 1879-1123. Available under: doi: 10.1007/s13361-011-0082-3

BibTex

@article{Susnea2011-04Bioma-18157,
  year={2011},
  doi={10.1007/s13361-011-0082-3},
  title={Biomarker candidates of Chlamydophila pneumoniae proteins and protein fragments identified by affinity-proteomics using FTICR-MS and LC-MS/MS},
  number={4},
  volume={22},
  issn={1044-0305},
  journal={Journal of The American Society for Mass Spectrometry},
  pages={784--788},
  author={Susnea, Iuliana and Bunk, Sebastian and Wendel, Albrecht and Hermann, Corinna and Przybylski, Michael}
}

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