@article{Micutkova2012-04-01Analy-18223,
  year={2012},
  doi={10.1002/ijc.26149},
  title={Analysis of the cellular uptake and nuclear delivery of insulin-like growth factor binding protein-3 in human osteosarcoma cells},
  number={7},
  volume={130},
  issn={0020-7136},
  journal={International Journal of Cancer},
  pages={1544--1557},
  author={Micutkova, Lucia and Hermann, Martin and Offterdinger, Martin and Hess, Michael W. and Matscheski, Andrea and Pircher, Haymo and Mück, Christoph and Ebner, Hannes-Leonhard and Laich, Andreas and Ferrando-May, Elisa and Zwerschke, Werner and Huber, Lukas A. and Jansen-Dürr, Pidder}
}

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    <dc:contributor>Offterdinger, Martin</dc:contributor>
    <dc:contributor>Laich, Andreas</dc:contributor>
    <dc:creator>Mück, Christoph</dc:creator>
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    <dc:creator>Ebner, Hannes-Leonhard</dc:creator>
    <dc:contributor>Matscheski, Andrea</dc:contributor>
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    <dc:creator>Offterdinger, Martin</dc:creator>
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    <dc:contributor>Mück, Christoph</dc:contributor>
    <dc:creator>Matscheski, Andrea</dc:creator>
    <dc:contributor>Jansen-Dürr, Pidder</dc:contributor>
    <dc:contributor>Hermann, Martin</dc:contributor>
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    <dcterms:bibliographicCitation>First publ. in: International Journal of Cancer ; 130 (2012), 7. - pp. 1544–1557</dcterms:bibliographicCitation>
    <dc:contributor>Zwerschke, Werner</dc:contributor>
    <dc:creator>Laich, Andreas</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-02-13T15:13:37Z</dc:date>
    <dc:contributor>Huber, Lukas A.</dc:contributor>
    <dc:contributor>Ferrando-May, Elisa</dc:contributor>
    <dc:creator>Pircher, Haymo</dc:creator>
    <dcterms:title>Analysis of the cellular uptake and nuclear delivery of insulin-like growth factor binding protein-3 in human osteosarcoma cells</dcterms:title>
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    <dc:creator>Jansen-Dürr, Pidder</dc:creator>
    <dc:creator>Ferrando-May, Elisa</dc:creator>
    <dcterms:abstract xml:lang="eng">Insulin-like growth factor (IGF) binding protein-3 (IGFBP-3) regulates cell proliferation and survival by extracellular interaction and inactivation of the growth factor IGF-I. Beyond that, IGF-independent actions mediated by intracellular IGFBP-3 including nuclear-IGFBP-3, have also been described. We here show, using both confocal and electron microscopy and cell fractionation, that the extracellular addition of IGFBP-3 to living cells results in rapid uptake and nuclear delivery of IGFBP-3, by yet partly unknown mechanisms. IGFBP-3 is internalized through a dynamin-dependent pathway, traffics through endocytic compartments and is finally delivered into the nucleus. We observed docking of IGFBP-3 containing structures to the nuclear envelope and found IGFBP-3 containing dot-like structures to permeate the nuclear envelope. In summary, our findings establish the pathway by which this tumor suppressor protein is delivered from extracellular space to the nucleus.</dcterms:abstract>
    <dc:creator>Hermann, Martin</dc:creator>
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    <dc:creator>Micutkova, Lucia</dc:creator>
    <dc:creator>Hess, Michael W.</dc:creator>
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    <dc:contributor>Pircher, Haymo</dc:contributor>
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    <dc:contributor>Micutkova, Lucia</dc:contributor>
    <dc:creator>Huber, Lukas A.</dc:creator>
    <dc:contributor>Hess, Michael W.</dc:contributor>
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    <dc:creator>Zwerschke, Werner</dc:creator>
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