Novel types of Ca2+ release channels participate in the secretory cycle of Paramecium cells

Ladenburger, Eva-Maria; Sehring, Ivonne; Korn, Iris; Plattner, Helmut

Novel types of Ca2+ release channels participate in the secretory cycle of Paramecium cells

Dateien

Plattner_Novel types.pdfGröße: 11 MBDownloads: 680

Datum

2009

Autor:innen

Ladenburger, Eva-Maria

Sehring, Ivonne

Korn, Iris

Plattner, Helmut

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Molecular and Cellular Biology. 2009, 29(13), pp. 3605-3622. ISSN 0270-7306. eISSN 1098-5549. Available under: doi: 10.1128/MCB.01592-08

Zusammenfassung

A database search of the Paramecium genome reveals 34 genes related to Ca2+-release channels of the inositol-1,4,5-trisphosphate (IP3) or ryanodine receptor type (IP3R, RyR). Phylogenetic analyses show that these Ca2+ release channels (CRCs) can be subdivided into six groups (Paramecium tetraurelia CRC-I to CRC-VI), each one with features in part reminiscent of IP3Rs and RyRs. We characterize here the P. tetraurelia CRC-IV-1 gene family, whose relationship to IP3Rs and RyRs is restricted to their C-terminal channel domain. CRC-IV-1 channels localize to cortical Ca2+ stores (alveolar sacs) and also to the endoplasmic reticulum. This is in contrast to a recently described true IP3 channel, a group II member (P. tetraurelia IP3RN-1), found associated with the contractile vacuole system. Silencing of either one of these CRCs results in reduced exocytosis of dense core vesicles (trichocysts), although for different reasons. Knockdown of P. tetraurelia IP3RN affects trichocyst biogenesis, while CRC-IV-1 channels are involved in signal transduction since silenced cells show an impaired release of Ca2+ from cortical stores in response to exocytotic stimuli. Our discovery of a range of CRCs in Paramecium indicates that protozoans already have evolved multiple ways for the use of Ca2+ as signaling molecule.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

LADENBURGER, Eva-Maria, Ivonne SEHRING, Iris KORN, Helmut PLATTNER, 2009. Novel types of Ca2+ release channels participate in the secretory cycle of Paramecium cells. In: Molecular and Cellular Biology. 2009, 29(13), pp. 3605-3622. ISSN 0270-7306. eISSN 1098-5549. Available under: doi: 10.1128/MCB.01592-08

BibTex

@article{Ladenburger2009-07Novel-18603,
  year={2009},
  doi={10.1128/MCB.01592-08},
  title={Novel types of Ca2+ release channels participate in the secretory cycle of Paramecium cells},
  number={13},
  volume={29},
  issn={0270-7306},
  journal={Molecular and Cellular Biology},
  pages={3605--3622},
  author={Ladenburger, Eva-Maria and Sehring, Ivonne and Korn, Iris and Plattner, Helmut}
}

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