Reggies/Flotillins regulate E-cadherin-mediated cell contact formation by affecting EGFR trafficking

Lade...
Vorschaubild
Dateien
Stuermer_191040.pdf
Stuermer_191040.pdfGröße: 6.33 MBDownloads: 441
Datum
2012
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Molecular Biology of the Cell. 2012, 23(10), pp. 1812-1825. ISSN 1059-1524. eISSN 1939-4586. Available under: doi: 10.1091/mbc.E11-12-1006
Zusammenfassung

The reggie/flotillin proteins were implicated in membrane trafficking and together with the cellular prion protein (PrP) in the recruitment of E-cadherin to cell contact sites. Here, we demonstrate that reggies as well as PrP downregulation in epithelial A431 cells caused overlapping processes and abnormal formation of adherens junctions (AJs). This defect in cell adhesion resulted from the reggie effects on Src tyrosine kinases and epidermal growth factor receptor (EGFR): loss of reggies reduced Src activation and EGFR phosphorylation at residues targeted by Src and c-cbl, and led to increased surface-exposure of EGFR by blocking its internalization. The prolonged EGFR signaling at the plasma membrane enhanced cell motility and macropinocytosis through which junction-associated E-cadherin is internalized and recycled back to AJs. Accordingly, blockage of EGFR signaling or macropinocytosis in reggie-deficient cells restored normal AJ formation. Thus, by promoting EGFR internalization, reggies restrict the EGFR signaling involved in E-cadherin macropinocytosis and recycling, regulate AJ formation and dynamics and thereby cell adhesion.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690SOLIS, Gonzalo, Yvonne SCHROCK, Nikola HÃœLSBUSCH, Marianne F. WIECHERS, Helmut PLATTNER, Claudia STÃœRMER, 2012. Reggies/Flotillins regulate E-cadherin-mediated cell contact formation by affecting EGFR trafficking. In: Molecular Biology of the Cell. 2012, 23(10), pp. 1812-1825. ISSN 1059-1524. eISSN 1939-4586. Available under: doi: 10.1091/mbc.E11-12-1006
BibTex
@article{Solis2012-05Reggi-19104,
  year={2012},
  doi={10.1091/mbc.E11-12-1006},
  title={Reggies/Flotillins regulate E-cadherin-mediated cell contact formation by affecting EGFR trafficking},
  number={10},
  volume={23},
  issn={1059-1524},
  journal={Molecular Biology of the Cell},
  pages={1812--1825},
  author={Solis, Gonzalo and Schrock, Yvonne and Hülsbusch, Nikola and Wiechers, Marianne F. and Plattner, Helmut and Stürmer, Claudia}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/19104">
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/19104/2/Stuermer_191040.pdf"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/19104/2/Stuermer_191040.pdf"/>
    <dc:creator>Stürmer, Claudia</dc:creator>
    <dc:creator>Hülsbusch, Nikola</dc:creator>
    <dc:language>eng</dc:language>
    <dc:contributor>Hülsbusch, Nikola</dc:contributor>
    <dcterms:title>Reggies/Flotillins regulate E-cadherin-mediated cell contact formation by affecting EGFR trafficking</dcterms:title>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Wiechers, Marianne F.</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Solis, Gonzalo</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-06-13T12:55:30Z</dcterms:available>
    <dcterms:bibliographicCitation>Publ. in: Molucular Biology of the Cell ; 23 (2012), 10. - S. 1812-1825</dcterms:bibliographicCitation>
    <dc:creator>Wiechers, Marianne F.</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/19104"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-06-13T12:55:30Z</dc:date>
    <dc:contributor>Plattner, Helmut</dc:contributor>
    <dcterms:issued>2012-05</dcterms:issued>
    <dc:creator>Plattner, Helmut</dc:creator>
    <dc:creator>Solis, Gonzalo</dc:creator>
    <dcterms:abstract xml:lang="eng">The reggie/flotillin proteins were implicated in membrane trafficking and together with the cellular prion protein (PrP) in the recruitment of E-cadherin to cell contact sites. Here, we demonstrate that reggies as well as PrP downregulation in epithelial A431 cells caused overlapping processes and abnormal formation of adherens junctions (AJs). This defect in cell adhesion resulted from the reggie effects on Src tyrosine kinases and epidermal growth factor receptor (EGFR): loss of reggies reduced Src activation and EGFR phosphorylation at residues targeted by Src and c-cbl, and led to increased surface-exposure of EGFR by blocking its internalization. The prolonged EGFR signaling at the plasma membrane enhanced cell motility and macropinocytosis through which junction-associated E-cadherin is internalized and recycled back to AJs. Accordingly, blockage of EGFR signaling or macropinocytosis in reggie-deficient cells restored normal AJ formation. Thus, by promoting EGFR internalization, reggies restrict the EGFR signaling involved in E-cadherin macropinocytosis and recycling, regulate AJ formation and dynamics and thereby cell adhesion.</dcterms:abstract>
    <dc:contributor>Stürmer, Claudia</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Schrock, Yvonne</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Schrock, Yvonne</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen