@article{Jakobs2019-05-27Engin-46455,
  year={2019},
  doi={10.3390/ijms20102597},
  title={Engineering of Nanobodies Recognizing the Human Chemokine Receptor CCR7},
  number={10},
  volume={20},
  journal={International Journal of Molecular Sciences},
  author={Jakobs, Barbara D. and Spannagel, Lisa and Purvanov, Vladimir and Uetz-von Allmen, Edith and Matti, Christoph and Legler, Daniel F.},
  note={Article Number: 2597}
}

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/46455">
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Spannagel, Lisa</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Uetz-von Allmen, Edith</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/46455"/>
    <dc:creator>Legler, Daniel F.</dc:creator>
    <dc:contributor>Purvanov, Vladimir</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46455/1/Jakobs_2-13zy0347kj6w93.pdf"/>
    <dcterms:issued>2019-05-27</dcterms:issued>
    <dc:creator>Uetz-von Allmen, Edith</dc:creator>
    <dc:contributor>Matti, Christoph</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:contributor>Jakobs, Barbara D.</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:creator>Jakobs, Barbara D.</dc:creator>
    <dc:contributor>Legler, Daniel F.</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-07-18T12:07:31Z</dcterms:available>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dcterms:title>Engineering of Nanobodies Recognizing the Human Chemokine Receptor CCR7</dcterms:title>
    <dc:creator>Purvanov, Vladimir</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46455/1/Jakobs_2-13zy0347kj6w93.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-07-18T12:07:31Z</dc:date>
    <dc:creator>Spannagel, Lisa</dc:creator>
    <dcterms:abstract xml:lang="eng">The chemokine receptor CCR7 plays a pivotal role in health and disease. In particular, CCR7 controls homing of antigen-bearing dendritic cells and T cells to lymph nodes, where adaptive immune responses are initiated. However, CCR7 also guides T cells to inflamed synovium and thereby contributes to rheumatoid arthritis and promotes cancer cell migration and metastasis formation. Nanobodies have recently emerged as versatile tools to study G-protein-coupled receptor functions and are being tested in diagnostics and therapeutics. In this study, we designed a strategy to engineer novel nanobodies recognizing human CCR7. We generated a nanobody library based on a solved crystal structure of the nanobody Nb80 recognizing the β&lt;sub&gt;2&lt;/sub&gt;-adrenergic receptor (β&lt;sub&gt;2&lt;/sub&gt;AR) and by specifically randomizing two segments within complementarity determining region 1 (CDR1) and CDR3 of Nb80 known to interact with β&lt;sub&gt;2&lt;/sub&gt;AR. We fused the nanobody library to one half of split-YFP in order to identify individual nanobody clones interacting with CCR7 fused to the other half of split-YFP using bimolecular fluorescence complementation. We present three novel nanobodies, termed Nb1, Nb5, and Nb38, that recognize human CCR7 without interfering with G-protein-coupling and downstream signaling. Moreover, we were able to follow CCR7 trafficking upon CCL19 triggering using Nb1, Nb5, and Nb38.</dcterms:abstract>
    <dc:creator>Matti, Christoph</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
  </rdf:Description>
</rdf:RDF>