Integration of Nodal and BMP Signaling by Mutual Signaling Effector Antagonism

Soh, Gary Huiming; Pomreinke, Autumn Penecilla; Müller, Patrick

Integration of Nodal and BMP Signaling by Mutual Signaling Effector Antagonism

Dateien

Soh_2-179kovc2gryeu5.pdfGröße: 4.29 MBDownloads: 66

Datum

2020

Autor:innen

Soh, Gary Huiming

Pomreinke, Autumn Penecilla

Müller, Patrick

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Cell Reports. Cell Press. 2020, 31(1), 107487. eISSN 2211-1247. Available under: doi: 10.1016/j.celrep.2020.03.051

Zusammenfassung

Opposing sources of bone morphogenetic protein (BMP) and Nodal signaling molecules are sufficient to induce the formation of a full axis in zebrafish embryos. To address how these signals orchestrate patterning, we transplant sources of fluorescently tagged Nodal and BMP into zebrafish embryos, robustly inducing the formation of secondary axes. Nodal and BMP signal non-cell-autonomously and form similar protein gradients in this context, but the signaling range of Nodal (pSmad2) is shorter than the BMP range (pSmad5). This yields a localized region of pSmad2 activity around the Nodal source, overlapping with a broad domain of pSmad5 activity across the embryo. Cell fates induced in various regions stereotypically correlate with pSmad2-to-pSmad5 ratios and can even be induced BMP- and Nodal-independently with different ratios of constitutively active Smad2 and Smad5. Strikingly, we find that Smad2 and Smad5 antagonize each other for specific cell fates, providing a mechanism for how cells integrate and discriminate between overlapping signals during development.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Nodal, BMP, zebrafish, embryo, development, Smad2, Smad5, gradients, axis induction

Zitieren

ISO 690

SOH, Gary Huiming, Autumn Penecilla POMREINKE, Patrick MÜLLER, 2020. Integration of Nodal and BMP Signaling by Mutual Signaling Effector Antagonism. In: Cell Reports. Cell Press. 2020, 31(1), 107487. eISSN 2211-1247. Available under: doi: 10.1016/j.celrep.2020.03.051

BibTex

@article{Soh2020Integ-55624,
  year={2020},
  doi={10.1016/j.celrep.2020.03.051},
  title={Integration of Nodal and BMP Signaling by Mutual Signaling Effector Antagonism},
  number={1},
  volume={31},
  journal={Cell Reports},
  author={Soh, Gary Huiming and Pomreinke, Autumn Penecilla and Müller, Patrick},
  note={Article Number: 107487}
}

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