An intermolecular FRET sensor detects the dynamics of T cell receptor clustering

Lade...
Vorschaubild
Dateien
Ma_2-1ds6ujn89egdb9.pdf
Ma_2-1ds6ujn89egdb9.pdfGröße: 3.5 MBDownloads: 237
Datum
2017
Autor:innen
Ma, Yuanqing
Pandzic, Elvis
Nicovich, Philip R.
Yamamoto, Yui
Kwiatek, Joanna
Pageon, Sophie V.
Benda, Aleš
Gaus, Katharina
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Gold
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Nature Communications. 2017, 8, 15100. eISSN 2041-1723. Available under: doi: 10.1038/ncomms15100
Zusammenfassung

Clustering of the T-cell receptor (TCR) is thought to initiate downstream signalling. However, the detection of protein clustering with high spatial and temporal resolution remains challenging. Here we establish a Förster resonance energy transfer (FRET) sensor, named CliF, which reports intermolecular associations of neighbouring proteins in live cells. A key advantage of the single-chain FRET sensor is that it can be combined with image correlation spectroscopy (ICS), single-particle tracking (SPT) and fluorescence lifetime imaging microscopy (FLIM). We test the sensor with a light-sensitive actuator that induces protein aggregation upon radiation with blue light. When applied to T cells, the sensor reveals that TCR triggering increases the number of dense TCR-CD3 clusters. Further, we find a correlation between cluster movement within the immunological synapse and cluster density. In conclusion, we develop a sensor that allows us to map the dynamics of protein clustering in live T cells.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690MA, Yuanqing, Elvis PANDZIC, Philip R. NICOVICH, Yui YAMAMOTO, Joanna KWIATEK, Sophie V. PAGEON, Aleš BENDA, Jérémie ROSSY, Katharina GAUS, 2017. An intermolecular FRET sensor detects the dynamics of T cell receptor clustering. In: Nature Communications. 2017, 8, 15100. eISSN 2041-1723. Available under: doi: 10.1038/ncomms15100
BibTex
@article{Ma2017-04-28inter-42983,
  year={2017},
  doi={10.1038/ncomms15100},
  title={An intermolecular FRET sensor detects the dynamics of T cell receptor clustering},
  volume={8},
  journal={Nature Communications},
  author={Ma, Yuanqing and Pandzic, Elvis and Nicovich, Philip R. and Yamamoto, Yui and Kwiatek, Joanna and Pageon, Sophie V. and Benda, Aleš and Rossy, Jérémie and Gaus, Katharina},
  note={Article Number: 15100}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/42983">
    <dc:contributor>Ma, Yuanqing</dc:contributor>
    <dc:creator>Nicovich, Philip R.</dc:creator>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Rossy, Jérémie</dc:creator>
    <dcterms:title>An intermolecular FRET sensor detects the dynamics of T cell receptor clustering</dcterms:title>
    <dc:contributor>Benda, Aleš</dc:contributor>
    <dcterms:abstract xml:lang="eng">Clustering of the T-cell receptor (TCR) is thought to initiate downstream signalling. However, the detection of protein clustering with high spatial and temporal resolution remains challenging. Here we establish a Förster resonance energy transfer (FRET) sensor, named CliF, which reports intermolecular associations of neighbouring proteins in live cells. A key advantage of the single-chain FRET sensor is that it can be combined with image correlation spectroscopy (ICS), single-particle tracking (SPT) and fluorescence lifetime imaging microscopy (FLIM). We test the sensor with a light-sensitive actuator that induces protein aggregation upon radiation with blue light. When applied to T cells, the sensor reveals that TCR triggering increases the number of dense TCR-CD3 clusters. Further, we find a correlation between cluster movement within the immunological synapse and cluster density. In conclusion, we develop a sensor that allows us to map the dynamics of protein clustering in live T cells.</dcterms:abstract>
    <dc:creator>Benda, Aleš</dc:creator>
    <dc:creator>Ma, Yuanqing</dc:creator>
    <dc:creator>Pandzic, Elvis</dc:creator>
    <dc:contributor>Pandzic, Elvis</dc:contributor>
    <dc:creator>Kwiatek, Joanna</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42983/1/Ma_2-1ds6ujn89egdb9.pdf"/>
    <dc:contributor>Rossy, Jérémie</dc:contributor>
    <dc:creator>Yamamoto, Yui</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42983"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-08-06T14:19:04Z</dcterms:available>
    <dc:creator>Pageon, Sophie V.</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:language>eng</dc:language>
    <dc:contributor>Kwiatek, Joanna</dc:contributor>
    <dc:contributor>Pageon, Sophie V.</dc:contributor>
    <dc:contributor>Nicovich, Philip R.</dc:contributor>
    <dc:contributor>Yamamoto, Yui</dc:contributor>
    <dc:creator>Gaus, Katharina</dc:creator>
    <dcterms:issued>2017-04-28</dcterms:issued>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42983/1/Ma_2-1ds6ujn89egdb9.pdf"/>
    <dc:contributor>Gaus, Katharina</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-08-06T14:19:04Z</dc:date>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Begutachtet
Ja
Diese Publikation teilen