The C. elegans TPR Containing Protein, TRD-1, Regulates Cell Fate Choice in the Developing Germ Line and Epidermis
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Correct cell fate choice is crucial in development. In post-embryonic development of the hermaphroditic Caenorhabitis elegans, distinct cell fates must be adopted in two diverse tissues. In the germline, stem cells adopt one of three possible fates: mitotic cell cycle, or gamete formation via meiosis, producing either sperm or oocytes. In the epidermis, the stem cell-like seam cells divide asymmetrically, with the daughters taking on either a proliferative (seam) or differentiated (hypodermal or neuronal) fate. We have isolated a novel conserved C. elegans tetratricopeptide repeat containing protein, TRD-1, which is essential for cell fate determination in both the germline and the developing epidermis and has homologs in other species, including humans (TTC27). We show that trd-1(RNAi) and mutant animals have fewer seam cells as a result of inappropriate differentiation towards the hypodermal fate. In the germline, trd-1 RNAi results in a strong masculinization phenotype, as well as defects in the mitosis to meiosis switch. Our data suggests that trd-1 acts downstream of tra-2 but upstream of fem-3 in the germline sex determination pathway, and exhibits a constellation of phenotypes in common with other Mog (masculinization of germline) mutants. Thus, trd-1 is a new player in both the somatic and germline cell fate determination machinery, suggestive of a novel molecular connection between the development of these two diverse tissues.
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HUGHES, Samantha, Henry WILKINSON, Sophie P. R. GILBERT, Marcia KISHIDA, Serena DING, Alison WOOLLARD, 2014. The C. elegans TPR Containing Protein, TRD-1, Regulates Cell Fate Choice in the Developing Germ Line and Epidermis. In: PLoS one. Public Library of Science (PLoS). 2014, 9(12), e114998. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0114998BibTex
@article{Hughes2014elega-54403, year={2014}, doi={10.1371/journal.pone.0114998}, title={The C. elegans TPR Containing Protein, TRD-1, Regulates Cell Fate Choice in the Developing Germ Line and Epidermis}, number={12}, volume={9}, journal={PLoS one}, author={Hughes, Samantha and Wilkinson, Henry and Gilbert, Sophie P. R. and Kishida, Marcia and Ding, Serena and Woollard, Alison}, note={Article Number: e114998} }
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