Partial Reactions of the Na,K-ATPase : Determination of Activation Energies and an Approach to Mechanism

Apell, Hans-Jürgen; Roudna, Milena

Partial Reactions of the Na,K-ATPase : Determination of Activation Energies and an Approach to Mechanism

Dateien

Apell_2-1ubzu4b1vg3mx6.pdfGröße: 938.25 KBDownloads: 200

Datum

2020

Open Access-Veröffentlichung

Open Access Hybrid

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

The Journal of Membrane Biology. Springer. 2020, 253(6), pp. 631-645. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s00232-020-00153-y

Zusammenfassung

Kinetic experiments were performed with preparations of kidney Na,K-ATPase in isolated membrane fragments or reconstituted in vesicles to obtain information of the activation energies under turnover conditions and for selected partial reactions of the Post-Albers pump cycle. The ion transport activities were detected with potential or conformation sensitive fluorescent dyes in steady-state or time-resolved experiments. The activation energies were derived from Arrhenius plots of measurements in the temperature range between 5 °C and 37 °C. The results were used to elaborate indications of the respective underlying rate-limiting reaction steps and allowed conclusions to be drawn about possible molecular reaction mechanisms. The observed consequent alteration between ligand-induced reaction and conformational relaxation steps when the Na,K-ATPase performs the pump cycle, together with constraints set by thermodynamic principles, provided restrictions which have to be met when mechanistic models are proposed. A model meeting such requirements is presented for discussion.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Sodium pump, Active ion transport, Post-Albers cycle, Reaction kinetics, Fluorescence, Molecular mechanism

Zitieren

ISO 690

APELL, Hans-Jürgen, Milena ROUDNA, 2020. Partial Reactions of the Na,K-ATPase : Determination of Activation Energies and an Approach to Mechanism. In: The Journal of Membrane Biology. Springer. 2020, 253(6), pp. 631-645. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s00232-020-00153-y

BibTex

@article{Apell2020-12Parti-52001,
  year={2020},
  doi={10.1007/s00232-020-00153-y},
  title={Partial Reactions of the Na,K-ATPase : Determination of Activation Energies and an Approach to Mechanism},
  number={6},
  volume={253},
  issn={0022-2631},
  journal={The Journal of Membrane Biology},
  pages={631--645},
  author={Apell, Hans-Jürgen and Roudna, Milena}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52001">
    <dcterms:issued>2020-12</dcterms:issued>
    <dc:language>eng</dc:language>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/52001/3/Apell_2-1ubzu4b1vg3mx6.pdf"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/52001/3/Apell_2-1ubzu4b1vg3mx6.pdf"/>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/52001"/>
    <dc:creator>Apell, Hans-Jürgen</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-12-02T08:22:05Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-12-02T08:22:05Z</dc:date>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Apell, Hans-Jürgen</dc:contributor>
    <dcterms:title>Partial Reactions of the Na,K-ATPase : Determination of Activation Energies and an Approach to Mechanism</dcterms:title>
    <dc:creator>Roudna, Milena</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Roudna, Milena</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dcterms:abstract xml:lang="eng">Kinetic experiments were performed with preparations of kidney Na,K-ATPase in isolated membrane fragments or reconstituted in vesicles to obtain information of the activation energies under turnover conditions and for selected partial reactions of the Post-Albers pump cycle. The ion transport activities were detected with potential or conformation sensitive fluorescent dyes in steady-state or time-resolved experiments. The activation energies were derived from Arrhenius plots of measurements in the temperature range between 5 °C and 37 °C. The results were used to elaborate indications of the respective underlying rate-limiting reaction steps and allowed conclusions to be drawn about possible molecular reaction mechanisms. The observed consequent alteration between ligand-induced reaction and conformational relaxation steps when the Na,K-ATPase performs the pump cycle, together with constraints set by thermodynamic principles, provided restrictions which have to be met when mechanistic models are proposed. A model meeting such requirements is presented for discussion.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja

Begutachtet

Ja