Strong Selection and High Mutation Supply Characterize Experimental Chlorovirus Evolution

Retel, Cas; Kowallik, Vienna; Becks, Lutz; Feulner, Philine G. D.

Strong Selection and High Mutation Supply Characterize Experimental Chlorovirus Evolution

Dateien

Retel_2-1w9l1b2ekvaey2.pdfGröße: 1.14 MBDownloads: 34

Datum

2022

Autor:innen

Retel, Cas

Kowallik, Vienna

Becks, Lutz

Feulner, Philine G. D.

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Virus Evolution. Oxford University Press. 2022, 8(1), veac003. ISSN 2057-1577. eISSN 2057-1577. Available under: doi: 10.1093/ve/veac003

Zusammenfassung

Characterizing how viruses evolve expands our understanding of the underlying fundamental processes, such as mutation, selection and drift. One group of viruses whose evolution has not yet been extensively studied are the Phycodnaviridae, a globally abundant family of aquatic large dsDNA viruses. Here we studied the evolutionary change of Paramecium bursaria Chlorella Virus 1 (PBCV-1) during experimental coevolution with its algal host. We used pooled genome sequencing of six independently evolved populations to characterize genomic change over five time points. Across six experimental replicates involving either strong or weak demographic fluctuations, we found single nucleotide polymorphisms (SNPs) at 67 sites. The occurrence of genetic variants was highly repeatable, with just two of the SNPs found in only a single experimental replicate. Three genes A122/123R, A140/145R and A540L showed an excess of variable sites, providing new information about potential targets of selection during Chlorella-Chlorovirus coevolution. Our data indicated that the studied populations were not mutation-limited and experienced strong positive selection. Our investigation highlighted relevant processes governing the evolution of aquatic large dsDNA viruses, which ultimately contributes to a better understanding of the functioning of natural aquatic ecosystems.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

virus evolution, genomics, repeatable genomic change, predicted phenotypic effect, Chlorovirus PBCV-1

Zitieren

ISO 690

RETEL, Cas, Vienna KOWALLIK, Lutz BECKS, Philine G. D. FEULNER, 2022. Strong Selection and High Mutation Supply Characterize Experimental Chlorovirus Evolution. In: Virus Evolution. Oxford University Press. 2022, 8(1), veac003. ISSN 2057-1577. eISSN 2057-1577. Available under: doi: 10.1093/ve/veac003

BibTex

@article{Retel2022-02-08Stron-56447,
  year={2022},
  doi={10.1093/ve/veac003},
  title={Strong Selection and High Mutation Supply Characterize Experimental Chlorovirus Evolution},
  number={1},
  volume={8},
  issn={2057-1577},
  journal={Virus Evolution},
  author={Retel, Cas and Kowallik, Vienna and Becks, Lutz and Feulner, Philine G. D.},
  note={Article Number: veac003}
}

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