Clustering of integrin β cytoplasmic domains triggers nascent adhesion formation and reveals a protozoan origin of the integrin-talin interaction

Baade, Timo; Paone, Christoph; Baldrich, Adrian; Hauck, Christof R.

Clustering of integrin β cytoplasmic domains triggers nascent adhesion formation and reveals a protozoan origin of the integrin-talin interaction

Dateien

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Datum

2019

Autor:innen

Baade, Timo

Paone, Christoph

Baldrich, Adrian

Hauck, Christof R.

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Scientific Reports. 2019, 9, 5728. eISSN 2045-2322. Available under: doi: 10.1038/s41598-019-42002-6

Zusammenfassung

Integrins and integrin-dependent cell-matrix adhesions are essential for a number of physiological processes. Integrin function is tightly regulated via binding of cytoplasmic proteins to integrin intracellular domains. Yet, the complexity of cell-matrix adhesions in mammals, with more than 150 core adhesome proteins, complicates the analysis of integrin-associated protein complexes. Interestingly, the evolutionary origin of integrins dates back before the transition from unicellular life to complex multicellular animals. Though unicellular relatives of metazoa have a less complex adhesome, nothing is known about the initial steps of integrin activation and adhesion complex assembly in protozoa. Therefore, we developed a minimal, microscope-based system using chimeric integrins to investigate receptor-proximal events during focal adhesion assembly. Clustering of the human integrin β1 tail led to recruitment of talin, kindlin, and paxillin and mutation of the known talin binding site abolished recruitment of this protein. Proteins indirectly linked to integrins, such as vinculin, migfilin, p130CAS, or zyxin were not enriched around the integrin β1 tail. With the exception of integrin β4 and integrin β8, the cytoplasmic domains of all human integrin β subunits supported talin binding. Likewise, the cytoplasmic domains of integrin β subunits expressed by the protozoan Capsaspora owczarzaki readily recruited talin and this interaction was based on an evolutionary conserved NPXY/F amino acid motif. The results we present here validate the use of our novel microscopic assay to uncover details of integrin-based protein-protein interactions in a cellular context and suggest that talin binding to integrin β cytoplasmic tails is an ancient feature of integrin regulation.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

BAADE, Timo, Christoph PAONE, Adrian BALDRICH, Christof R. HAUCK, 2019. Clustering of integrin β cytoplasmic domains triggers nascent adhesion formation and reveals a protozoan origin of the integrin-talin interaction. In: Scientific Reports. 2019, 9, 5728. eISSN 2045-2322. Available under: doi: 10.1038/s41598-019-42002-6

BibTex

@article{Baade2019-04-05Clust-45732,
  year={2019},
  doi={10.1038/s41598-019-42002-6},
  title={Clustering of integrin β cytoplasmic domains triggers nascent adhesion formation and reveals a protozoan origin of the integrin-talin interaction},
  volume={9},
  journal={Scientific Reports},
  author={Baade, Timo and Paone, Christoph and Baldrich, Adrian and Hauck, Christof R.},
  note={Article Number: 5728}
}

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