Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
In summary, the results of this dissertation show that autofluorescence is a remarkable property of plastics and that it experiences an increase in intensity when incubation is performed at high temperatures. This increased intensity of fluorescence is only partially sufficient for modern methodology and is more suitable for detection or contamination prediction. The integration of the temporal resolution of these fluorescence processes allows an extension to perform a specific characterization, as well as differentiation of the micro- and nanoplastic. The combination of mean intensity-weighted τ and mean amplitude-weighted τ values allowed, under the given settings, the significant differentiation of 94.55 % of the included plastics with and without elevated temperature treatment. Phasor analysis speeds up the measurement, allows analysis of a larger amount of image data (high throughput), and reduces from bias by avoiding fitting algorithms and enhanced the analysis with helpful interactive tools. Applicability in more complex matrices or in deeper tissues could not be realized at this stage and needs follow-up experiments. Furthermore, FLIM in combination with phasor analysis showed the potential for submicron analysis of plastics, 3D sections of tissue and environmental samples. Furthermore, technologies such as multiphoton FLIM, STED-FLIM and new algorithms for image analysis are available for further experiments and developments.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
MONTELEONE, Adrian, 2021. Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes [Dissertation]. Konstanz: University of KonstanzBibTex
@phdthesis{Monteleone2021Devel-54864,
year={2021},
title={Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes},
author={Monteleone, Adrian},
address={Konstanz},
school={Universität Konstanz}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/54864">
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/54864/3/Monteleone_2-1xtejs8gkw9ri5.pdf"/>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-09-13T09:37:34Z</dc:date>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-09-13T09:37:34Z</dcterms:available>
<dc:contributor>Monteleone, Adrian</dc:contributor>
<dc:rights>terms-of-use</dc:rights>
<dc:creator>Monteleone, Adrian</dc:creator>
<dcterms:title>Development of a novel method for the identification and differentiation of micro- and nanoplastics based on time resolved fluorescence processes</dcterms:title>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dcterms:abstract xml:lang="eng">In summary, the results of this dissertation show that autofluorescence is a remarkable property of plastics and that it experiences an increase in intensity when incubation is performed at high temperatures. This increased intensity of fluorescence is only partially sufficient for modern methodology and is more suitable for detection or contamination prediction. The integration of the temporal resolution of these fluorescence processes allows an extension to perform a specific characterization, as well as differentiation of the micro- and nanoplastic. The combination of mean intensity-weighted τ and mean amplitude-weighted τ values allowed, under the given settings, the significant differentiation of 94.55 % of the included plastics with and without elevated temperature treatment. Phasor analysis speeds up the measurement, allows analysis of a larger amount of image data (high throughput), and reduces from bias by avoiding fitting algorithms and enhanced the analysis with helpful interactive tools. Applicability in more complex matrices or in deeper tissues could not be realized at this stage and needs follow-up experiments. Furthermore, FLIM in combination with phasor analysis showed the potential for submicron analysis of plastics, 3D sections of tissue and environmental samples. Furthermore, technologies such as multiphoton FLIM, STED-FLIM and new algorithms for image analysis are available for further experiments and developments.</dcterms:abstract>
<dcterms:issued>2021</dcterms:issued>
<bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/54864"/>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dc:language>eng</dc:language>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/54864/3/Monteleone_2-1xtejs8gkw9ri5.pdf"/>
</rdf:Description>
</rdf:RDF>
